德保矮马和蒙古马体尺发育相关lncRNAs的筛选和比较  

Screening and Comparison of LncRNAs Related to Body Size Development in Debao Pony(Equus caballus)and Mongolian Horse

作　　者：房君[1] 凌宇[1] 潘静[1] 曹宇[1] 周欢敏[1] 张焱如[1] FANG Jun;LING Yu;PAN Jing;CAO Yu;ZHOU Huan-Min;ZHANG Yan-Ru(College of Life Sciences,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018

出　　处：《农业生物技术学报》2020年第8期1417-1430,共14页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31560621)。

摘　　要：德保矮马(Equus caballus)是我国广西的优秀马品种,在体高方面与我国其他品种马匹有显著差异,但对德保矮马体尺发育的调控机制知之甚少.本研究对蒙古马和德保矮马在幼年和成年时期的垂体和长骨骨骺进行组织样本采集和转录组测序,得到不同发育阶段差异表达的长链非编码RNAs(long non-coding RNA,lncRNAs)847个,其中512个上调表达、335个下调表达;得到不同品种间差异表达的lncRNAs 1633个,其中882个上调表达、751个下调表达;另外,德保矮马成年和幼年发育时期垂体组织中差异表达的lncRNAs为41个,而长骨组织中为5个;蒙古马成年和幼年发育时期垂体组织中差异表达的lncRNAs为41个,而长骨组织中为7个;在幼年时期蒙古马和德保矮马垂体组织中差异表达的lncRNAs为59个,而长骨组织中为34个;在成年时期蒙古马和德保矮马垂体组织中差异表达的lncRNAs为32个,而长骨组织中为35个.将这些差异表达lncRNAs所对应的靶基因进行KEGG和GO(Gene Ontology)分析,结果主要涉及神经系统和内分泌系统发育等方面.根据差异表达基因相关的基因功能富集、通路富集和前人研究结果,共筛选出20个生长发育相关蛋白的编码基因,其中差异表达的lncRNAs中有13个是通过反式作用分别靶向生长激素(growth hormone,GH)和胰岛素样生长因子1(insulin-like growth factor-1,IGF-1)基因.本研究结果可为德保矮马体尺发育相关机制研究及靶向矮小性状的家畜育种提供参考依据.Debao pony(Equus caballus)is an excellent horse breed in Guangxi of China.It has significant differences in body height compared with other breeds of horses in China.However,the regulation mechanism of body height growth of Debao pony is still poorly understood.This study was carried out from sampling and transcriptome sequencing of Mongolian horse and Debao pony childhood and adulthood pituitary and long bone epiphysis.There were 847 long-chain non coding RNAs(lncRNAs)differentially expressed at different stages of development,including 512 up-regulated and 335 down-regulated;and 1633 lncRNAs were differentially expressed in different varieties,including 882 up-regulated and 751 down-regulated.In addition,there were 41 lncRNAs differentially expressed in pituitary tissue and 5 lncRNAs in long bone tissue during adult and juvenile development period of Debao pony;41 lncRNAs were differentially expressed in pituitary tissue and 7 lncRNAs in long bone during the two development period of Mongolian horse.There were 59 lncRNAs differentially expressed in pituitary tissues and 34 lncRNAs in long bone tissues during juvenile period of Mongolian horse and Debao pony;32 lncRNAs were differentially expressed in pituitary tissues and 35 lncRNAs in long bone tissue during adult period of the two horse breeds.KEGG and Gene Ontology(GO)analyses were performed on the corresponding target genes of these differentially expressed lncRNAs,and the results showed that they were mainly involved in the development of nervous system and endocrine system.According to the function enrichment,pathway enrichment and previous studies of key candidate genes,20 protein coding genes were screened out,and 13 differentially expressed lncRNAs targeted growth hormone(GH)and insulin-like growth factor-1(IGF-1)genes by trans action.The results of this study could provide a reference for the study on the mechanism of Debao pony body size development and the breeding of domestic animals targeting dwarfism.

关 键 词：德保矮马 垂体 长骨骨骺 转录组测序(RNA-seq) 长链非编码RNA(lncRNA) 

分 类 号：S821.81[农业科学—畜牧学]