新疆野核桃种质对低温胁迫的生理响应  

Physiological response of Xinjiang wild walnut germplasm to low temperature stress

作　　者：张敏欢 王建成[2] 杨红兰[2] 张道远[2] 邓鹏程 崔志军 ZHANG Min-huan;WANG Jian-cheng;YANG Hong-lan;ZHANG Dao-yuan;DENG Peng-cheng;CUI Zhi-jun(College of Life Sciences,Shihezi University,Shihezi 832003,Xinjiang,China;State Key Laboratory of Desert and Oasis Ecology,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China;College of Agriculture,Shihezi University,Shihezi 832003,Xinjiang,China;Gongliu County Forestry and Steppe Bureau,Gongliu 835400,Xinjiang,China)

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]中国科学院新疆生态与地理研究所荒漠与绿洲生态国家重点实验室,乌鲁木齐830011 [3]石河子大学农学院,新疆石河子832003 [4]巩留县林业和草原局,新疆巩留835400

出　　处：《应用生态学报》2020年第8期2558-2566,共9页Chinese Journal of Applied Ecology

基　　金：陕西省科学院科技计划项目(2019k-07);中国科学院西部之光团队项目(2018-XBJCTD-001)资助。

摘　　要：以新疆巩留野核桃林37份新疆野核桃种质资源的1年生枝条为材料,6份核桃楸为对照,利用人工气候箱模拟春季低温,分别于-20℃和4℃处理12 h,测定其相对电导率、游离脯氨酸、可溶性糖、丙二醛含量、过氧化物酶活性等生理生化指标,并进行相关分析。采用隶属函数和主成分分析综合评价核桃种质对低温的生理响应。结果表明:低温胁迫下,新疆野核桃相对电导率、游离脯氨酸含量、可溶性糖含量、丙二醛含量和过氧化物酶活性均呈上升趋势。综合评价其低温抗性与生境相关:巩留野核桃林中沟>东沟>西沟>主沟。新疆野核桃表现出比核桃楸更为耐低温的特性。本研究从37份新疆野核桃种质中筛选出7份耐低温种质,为改良核桃品种和提高核桃耐倒春寒等生长期的突然天气变化的能力提供科学参考。We examined the physiological response of 1-year-old branches of 37 Xinjiang wild walnut germplasm resources in Gongliu wild walnut forest under cold temperatures(-20℃and 4℃)for 12 hours,compared the responses with that of Juglans mandshurica.The relative conductivity(REC),free proline(PRO),soluble sugar(SS),malondialdehyde(MDA)content and peroxidase(POD)activity were measured in an artificial climate chamber by simulating spring low temperature.Subordinate function and principal component analysis were used to evaluate the physiological response of walnut germplasm to low temperature.The results showed that the REC,PRO,SS,MDA content and POD activity of Xinjiang wild walnut were increased.By evaluating the relationship between low temperature resistance and habitat,we found that cold tolerance level was middle valley>east valley>west valley>general valley.Xinjiang wild walnut showed stronger cold tolerance than J.mandshurica.Seven germplasm with cold resistance were selected from the total 37 wild walnut germplasm in Xinjiang,which provided a reference for improving walnut varieties and their responses to sudden weather change in late spring and other growing stages.

关 键 词：新疆野核桃 枝条 低温 种质资源 

分 类 号：S664.1[农业科学—果树学]