植茶年限对土壤微生物群落结构及多样性的影响  

Effects of different tea plantation ages on soil microbial community structure and diversity

作　　者：姚泽秀 李永春[1] 李永夫[1] 陈志豪[1] YAO Ze-xiu;LI Yong-chun;LI Yong-fu;CHEN Zhi-hao(School of Environmental and Resource Sciences,Zhejiang Agriculture and Forestry University/Zhejiang Provincial Key Laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration/State Key Laboratory of Subtropical Silvicul-ture,Hangzhou 311300,China)

机构地区：[1]浙江农林大学环境与资源学院/浙江省森林生态系统碳循环与固碳减排重点实验室/亚热带森林培育国家重点实验室,杭州311300

出　　处：《应用生态学报》2020年第8期2749-2758,共10页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(31670618)资助。

摘　　要：为探明植茶年限对土壤微生物群落结构及多样性的影响,以0、20、25、38和48年茶园土壤表层(0~20 cm)、亚表层(20~40 cm)土壤样品为研究对象,采用T-RFLP技术及qPCR方法对土壤细菌(B)、真菌(F)群落进行分析。结果表明:植茶后土壤理化性质明显改变,随植茶年限的增加土壤有机碳、碱解氮及有效磷含量呈先升高后降低的趋势,表层土壤有机碳和全氮含量均显著高于亚表层土壤。不同植茶年限土壤细菌群落组分存在差异且多样性指数随植茶年限的增加呈下降趋势,而不同植茶年限土壤真菌群落组分差异不明显且多样性指数无显著差异。总体来看,土壤细菌群落对植茶年限的响应比真菌群落敏感。随植茶年限的增加,茶园土壤微生物群落有从F/B较低的"细菌型"向F/B较高的"真菌型"转变的趋势。We investigated the effects of tea plantation age on soil microbial community structure and diversity with surface and subsurface soil samples(0-20 and 20-40 cm)from tea plantation at different ages(0,20,25,38 and 48 years).We analyzed soil bacterial and fungal communities by terminal restriction fragment length polymorphism(T-RFLP)and real-time quantitative PCR techniques.The results showed that soil physicochemical properties changed significantly after planting tea.The contents of soil organic carbon,available nitrogen,and available phosphorus increased at first,and gradually decreased with the increases of tea plantation age.The contents of organic carbon and total nitrogen in the topsoil were significantly higher than those in the subsoil.Moreover,soil bacterial community composition varied across tea plantation age.Bacterial diversity index decreased with increasing tea plantation age.The composition and diversity of soil fungal communities did not show a clear relationship with the tea plantation age.Overall,soil bacterial communities was more sensitive to the tea plantation age than fungal communities.With the increases of tea plantation age,the ratio of soil fungi to bacteria showed increasing trend.Soil microbial community in the tea plantation changed from a"bacterial type"with low fungi/bacteria ratio(F/B)to a"fungal type"with high F/B.

关 键 词：植茶年限 土壤细菌 土壤真菌 土层 群落特征 

分 类 号：S154.3[农业科学—土壤学] S571.1[农业科学—农业基础科学]