核桃果皮中单宁、总黄酮和茶多酚的提取工艺研究  

Simultaneous extraction of tannin,total flavonoids and tea polyphenol from Juglans regia L.husk

作　　者：王吉平 何铁光[1] 苏利荣[1] 秦芳[1] 李琴[1] 苏天明[1] 曾成城 张雨[2] 徐亮[2] 滕美珍 Wang Jiping;He Tieguang;Su Lirong;Qin Fang;Li Qin;Su Tianming;Zeng Chengcheng;Zhang Yu;Xu Liang;Teng Meizhen(Agricultural Resources and Environment Research Institute,Guangxi Academy of Agricultural Sciences,Nanning Guangxi 530007,China;Yunnan Academy of Forestry,Kunming Yunnan 650201,China;Guangxi Fengshan Jiahong seedling Co.,Ltd,Hechi Guangxi 574600,China.)

机构地区：[1]广西农业科学院农业资源与环境研究所,广西南宁530007 [2]云南省林业科学院,云南昆明650201 [3]广西凤山县佳弘种苗有限公司,广西河池574600

出　　处：《南方林业科学》2020年第4期30-33,共4页South China Forestry Science

基　　金：广西创新驱动发展专项项目(项目编号:桂科AA17204058-14);广西农业科学院基本科研业务费项目(项目编号:桂农科2018YT39);广西农业科学院基本科研业务费项目(项目编号:桂农科2018YT40)。

摘　　要：核桃果皮中的抑菌成分降低了其堆肥腐熟效率。为实现核桃果皮的肥料化,对核桃果皮中抑菌成分的提取工艺进行探究。试验采用常温、50℃、60℃、70℃的水和50%、70%、90%的乙醇在料液比1:2的条件下,分别浸提1 h和3 h,对浸提液中单宁、总黄酮和茶多酚的含量进行检测分析,探究提取核桃果皮中抑菌成分的最佳提取条件。水温70℃下浸提3 h下得到的3种物质的得率最高,其中核桃青果皮的单宁得率为3.34 g·kg-1,总黄酮得率为1.29 g·kg-1,茶多酚得率为3.18 g·kg-1,核桃黑果皮的单宁得率为1.69 g·kg-1,总黄酮得率为0.90 g·kg-1,茶多酚得率为1.71 g·kg-1。该方法可有效减少核桃果皮中的抑菌成分,为核桃果皮堆肥提供一种简便高效的预处理工艺。The antimicrobial substances in Juglans regia L.husk reduced its efficiency of compost.Therefore,the extraction process of antimicrobial substances in J.regia L.husk was studied for realizing the fertilization of J.regia L.husk.Room temperature,50℃,60℃and 70℃water and 50%,70%and 90%ethanol were set in the experiment and solid-liquid ratio was 1:2,so as to research the optimal extraction conditions,the content of antimicrobial substances was detected and analyzed after 1 h and 3 h extraction respectively.The yield of the three substances was the highest after extraction at 70℃water for 3 h,among which the yield of tannin、total flavonoids and tea polyphenol was 3.34 g·kg-1,1.29 g·kg-1,and 3.18 g·kg-1 from J.regia L.green husk,and 1.69 g·kg-1,0.90 g·kg-1and 1.71 g·kg-1 from J.regia L.black husk.The method can effectively reduce the antibacterial components in J.regia L.husk and provide a simple and efficient pretreatment process for J.regia L.husk composting.

关 键 词：核桃果皮 抑菌成分 提取工艺 堆肥预处理 

分 类 号：S792.13[农业科学—林木遗传育种] TQ914.3[农业科学—林学]