微小小单胞菌ATCC33270脂磷壁酸致炎作用的研究  

The inflammation induced by Parvimonas micra ATCC33270 lipoteichoic acid

作　　者：刘琨[1] 侯本祥[1] LIU Kun;HOU Ben-xiang(Department of Endodontics,Capital Medical University School of Stomatology,Beijing 100050,China)

机构地区：[1]首都医科大学口腔医学院牙体牙髓科,北京100050

出　　处：《北京口腔医学》2020年第4期208-212,共5页Beijing Journal of Stomatology

摘　　要：目的探讨微小小单胞菌(P. micra)ATCC33270脂磷壁酸(LTA)的致炎作用。方法使用丁醇和疏水交换层析柱法进行P. micra LTA的提纯,将P. micra LTA作用于小鼠巨噬细胞,观察炎性细胞因子的表达。结果成功提纯P. micra LTA,将P. micra LTA作用在小鼠巨噬细胞上,发现随着LTA浓度的升高,TNF-α和NO的表达均增强,具有浓度相关性。结论小鼠巨噬细胞对P. micra LTA的刺激产生明显反炎症应,P. micra LTA具有致炎的生物学活性。Objective To investigate the inflammatory effect of Parvimonas micra(P.micra)ATCC33270 lipoteichoic acid(LTA).Methods P.micra LTA was purified by butanol and hydrophobic exchange chromatography.P.micra LTA was treated on mouse macrophages to observe the expression of inflammatory cytokines.Results P.micra LTA was successfully purified and the expression of TNF-αand NO was observed.The results showed that the expression of TNF-αand NO increased with the increase of LTA concentration in a concentration-dependent manner.Conclusion P.micra LTA had the biological activity of causing inflammation.

关 键 词：微小小单胞菌 脂磷壁酸 致炎作用 

分 类 号：R378.8[医药卫生—病原生物学]