基于多元统计学方法的亚香棒虫草与冬虫夏草化学差异研究  

Analysis of chemical differences between Cordyceps hawkesii and Cordyceps sinensis based on multivariate statistics

作　　者：潘宏春 阳勇[2] 秦伟瀚[2] 李卿[2] 雷中惠 PAN Hong-chun;YANG Yong;QIN Wei-han;LI Qing;LEI Zhong-hui(Chongqing Traditional Herbal Pieces of Decoction Factory Co.,Ltd.,Chongqing 408000,China;Chonging Academy of Chinese Materia Medica,Chongqing 400065,China;Department of Pharmacy,Chongqing Changshou District People’s Hospital,Chongqing 401220,China)

机构地区：[1]重庆中药饮片厂有限公司,重庆408000 [2]重庆市中药研究院,重庆400065 [3]重庆市长寿区人民医院药学部,重庆401220

出　　处：《中草药》2020年第16期4316-4324,共9页Chinese Traditional and Herbal Drugs

基　　金：国家重大新药创制项目(2017ZX09101002-002-004);重庆市科技局项目(cstc2019jxjl-jbky0017);重庆市自然基金项目(cstc2019jcyj-msxmX0464)。

摘　　要：目的采用高分辨质谱分析鉴定亚香棒虫草Cordycepshawkesii与冬虫夏草Cordycepssinensis间差异性成分。方法结合PeakView、SIMCA-P等软件、在线数据库(Human Metabolome Database、Pub Chem、Metlin)及二级碎片裂解规律分析鉴定区分2种虫草的显著差异性成分。结果通过正交偏最小二乘判别分析(OPLS-DA)发现区分亚香棒虫草与冬虫夏草的显著差异性成分共12个,其中11个为氨基酸及其衍生物以及1个磷脂酰胆碱。结论通过差异分析并未鉴定出如虫草素、腺苷等特征成分,从化学层面证明了亚香棒虫草或可作为冬虫夏草的补充资源,为亚香棒虫草深入开发应用提供科学支撑。Objective To analyze and identify the significant different components between Cordyceps hawkesii and Cordyceps sinensis by using method of ultra performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS). Methods Mass spectrometry combined with formula finder of PeakView software and database(Human Metabolome Database, Pub Chem, Metlin) and secondary fragmentation analysis, significant different components were identified and analyzed. Results Through OPLS-DA analysis, it was found that 12 significant different components were identified. Eleven of them were amino acids and their metabolites, and one was phosphatidylcholine. Conclusion Surprisingly, characteristic components such as cordycepin and adenosine were not identified by significant difference analysis. In this study, it was proved that C. hawkesii can be used as a supplementary resource instead of C. sinensis, which provided scientific support for the further development and utilization of C. hawkesii.

关 键 词：亚香棒虫草 冬虫夏草 超高效液相色谱-四极杆-飞行时间质谱 正交偏最小二乘判别分析 差异成分 

分 类 号：R282.6[医药卫生—中药学]