白花丹参水提取物通过调控NOD2基因表达对高糖诱导小鼠肾脏足细胞损伤的保护作用及其机制研究  

The water extract of Salvia miltorrhiza f.alba protects renal podocyte from high glucose-inducedinjury by regulating NOD2 genein mice

作　　者：段荣[1] 夏林 蒋健[1] 杨志忠[1] 郑晓玉 DUAN Rong;XIA Lin;JIANG Jian;YANG Zhizhong;ZHENG Xiaoyu(Department of Kidney Medicine,First People’s Hospital of Neijiang City,Neijiang 641000,China)

机构地区：[1]内江市第一人民医院肾内科,641000

出　　处：《免疫学杂志》2020年第9期777-783,共7页Immunological Journal

摘　　要：目的研究白花丹参水提取物对高糖诱导的小鼠肾脏足细胞MPC5损伤的影响及分子作用机制。方法高糖刺激和白花丹参水提取物处理MPC5细胞,qRT-PCR检测NOD2、podocin和nephrin mRNA表达,Western blot检测NOD2、podocin和nephrin蛋白表达,流式细胞术测定细胞凋亡率。结果NOD2在大多数人糖尿病肾病组织中呈高表达;高糖抑制小鼠足细胞MPC5中podocin和nephrin的表达,促进MPC5细胞凋亡,诱导MPC5细胞中NOD2 mRNA和蛋白的表达;白花丹参水提取物可以促进高糖诱导的MPC5细胞中podocin和nephrin的表达,抑制NOD2基因的表达,抑制高糖诱导的MPC5细胞凋亡,抑制NOD2表达可促进高糖诱导的MPC5细胞中podocin和nephrin的表达,并抑制细胞凋亡;过表达NOD2逆转了白花丹参水提取物对高糖诱导的MPC5细胞损伤的作用。结论白花丹参水提取物可能通过调控NOD2基因表达减轻高糖诱导的小鼠肾脏足细胞MPC5的损伤,抑制细胞凋亡。白花丹参水提取物对糖尿病肾病具有潜在治疗作用。Recent studies have revealed that the water extract of Salvia miltiorrhiza f.alba(SMA)could protect the renal function of diabetic nephropathy rats.While this study was designed to investigate the effect of SMA on high glucose-induced renal podocyte MPC5 injury in mice and its molecular mechanism.The MPC5 cells were cultured and differentiated,then treated with high-glucose and/or SMA.The mRNA and protein expression levels of NOD2,podocin and nephrin were detected by qRT-PCR and Western blotting;the cell apoptotic rate was determined by flow cytometry.Data showed that NOD2 was highly expressed in most diabetic nephropathy tissues.High glucose inhibited the expression of podocin and nephrin,promoted MPC5apoptosis and induced the expression of NOD2 mRNA and protein in mouse podocyte MPC5.SMA promoted the expression of podocin and nephrin,inhibited the expression of NOD2 gene and inhibited the apoptosis of MPC5 cells induced by high glucose.Inhibition of NOD2 gene promoted the expression of podocin and nephrin,while inhibited the apoptosis in MPC5 cells induced by high glucose.Overexpression of NOD2 reversed the effects of SMA on high glucosestimulatedMPC5 cells.Taken together,SMA alleviates the injury of MPC5 induced by high glucose and inhibitscell apoptosis by regulating NOD2 gene expression.Therefore,SMA has potential therapeutic effect on diabetic nephropathy.

关 键 词：白花丹参 高糖 小鼠肾脏足细胞 MPC5 NOD2 凋亡 

分 类 号：R587.2[医药卫生—内分泌]