雷公藤多苷片对糖尿病肾病大鼠肾组织RhoA/ROCK1表达的影响  

Effects of Tripeygium Wilfordi Multiglucoside Tablets on Expressions of RhoA and ROCK1 in Renal Tissue of Rats with Diabetic Nephropathy

作　　者：宋纯东[1] 宋丹 任献青[1] 翟文生[1] 丁樱[1] SONG Chundong;SONG Dan;REN Xianqing;ZHAI Wensheng;DING Ying(The First Affiliated Hospital of Henan University of Traditional Chinese,Zhengzhou 450008,Henan,China;Henan University of Traditional Chinese Medicine,Zhengzhou 450046,Henan,China)

机构地区：[1]河南中医药大学第一附属医院,河南郑州450008 [2]河南中医药大学,河南郑州450046

出　　处：《中华中医药学刊》2020年第8期166-169,I0034,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：全国第四批中医优秀人才临床人才培养项目(国中医药人教发[2017]124);丁樱全国名老中医药专家传承工作室项目(2100601-CZ0138);河南省中医管理局国家中医临床研究基地科研专项重点项目(2018JDZX119);河南省中医管理局国家中医临床研究基地科研专项普通项目(2018JDZX017)。

摘　　要：目的探讨雷公藤多苷片(TW)对糖尿病肾病(DN)大鼠肾组织RhoA/ROCK1表达的影响。方法SD雄性大鼠随机分为空白组、模型组、TW组。空白组和模型组灌服生理盐水,TW组给予TW片。8周后测体质量、肾质量、肥大指数及肾小球体积;生化检测24 h尿蛋白、血清白蛋白(ALB)、血脂(TC、TG)、空腹血糖(FBG)等;过碘酸-雪夫氏(PAS)染色观察肾脏病理;酶联免疫吸附测定(ELISA)法检测肾组织RhoA、ROCK1的浓度值。结果模型组ALB减少,体质量、肥大指数、TG、TC及FBG升高,肾小球体积增大,RhoA、ROCK1的表达增多,与空白组比较(P<0.05或P<0.01),具有统计学差异,说明造模成功。TW干预后,RhoA、ROCK1的表达下调,24 h尿蛋白减少,ALB升高,血脂、血糖及肥大指数降低,肾小球直径缩小,大鼠体质量及肾脏病理改善,足细胞损害减轻与模型组比较(P<0.05或P<0.01),具有统计学意义。结论TW可能通过影响DN大鼠肾组织RhoA、ROCK1的表达,而改善上述生化指标,减轻肾脏病理损害,且对肝、肾功能未见明显损害。Objective To investigate the effects of tripeygium wilfordi multiglucoside tablets on the expressions of RhoA and Rock1 in renal tissue of rats with diabetic nephropathy.Methods SD rats were randomly divided in to 3 groups:blank group,model group and TW group.The blank group and model group were given normal saline by gavage.TW group were given TW by gavage.The weight of rat,weight of kidney,hypertrophy index and the glomerular volume were measured eight weeks later.In addition,some indicators such as the quantity of 24 h uninary protein,serum albumin(ALB),serum total cholesterol(TC),triglyceride(TG)and fasting blood glucose(FBG)were determined by means of biochemical analysis eight weeks later.Renal pathology was observed by periodic acid-schiefs reaction(PAS).The enzyme linked immunosorbent assay(ELISA)was used to measure the levels of RhoA and ROCK1 in rat renal tissue.Results TW could down-regulate the expressions of RhoA and Rock1 and increase the 24 h urinary protein.TW could increase the level of albumin(ALB)and decrease the blood liquid,blood glucose and hypertrophy index.TW could also shorten the glomerular diameter and improve the weight of the rats and the renal pathology and could reduce the damage of podocytes.Conclusion TW could regulate the expressions of RhoA and Rock1 in renal tissue of rats with diabetic nephropathy,which is likely to improve some biochemical indicators related to renal pathological damage.In addition,there is no obvious damage to liver and kidney function.

关 键 词：糖尿病肾病 雷公藤多苷 RhoA/ROCK1 实验研究 

分 类 号：R285.5[医药卫生—中药学]