LPS联合烟熏致COPD大鼠模型的证候判定  

Syndrome Judgment of COPD Rat Model Induced by LPS Combined with Smoking

作　　者：王智超 吴雨潇 王振兴[1] 刘丽耘[2] 张秀 李光 王飞[1] Wang Zhichao;Wu Yuxiao;Wang Zhenxing;Liu Liyun;Zhang xiu;Li Guang;Wang Fei(School of Clinical Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Internatonal education college,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Qujing Hospital of Traditional Chinese Medicine,Chengdu 610075,China)

机构地区：[1]成都中医药大学临床医学院,成都610075 [2]成都中医药大学国际教育学院,成都610075 [3]曲靖市中医医院,曲靖655000

出　　处：《世界科学技术-中医药现代化》2020年第6期2035-2041,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：四川省科学技术厅重大课题(2015JY0267)补肺汤调控TLR/NF-KB信号转导通路改善COPD气道重塑机制研究,负责人:王飞;云南省科学技术厅中医联合专项(2017FF116-048)平喘化痰膏治疗慢性阻塞性肺疾病的机制探讨,负责人:李光;成都中医药大学科学技术发展基金(ZRQN1562):基于中医四诊客观化诊断技术辨识COPD稳定期患者中医体质分布规律与中医证候的相关性研究,负责人:刘丽耘。

摘　　要：目的从大鼠宏观表征定量化分析入手,以"因脉证治"为探索思路,分析LPS联合烟熏造成大鼠慢性阻塞性肺疾病的证候归属。方法将SD大鼠随机分为空白组和模型组。模型组采用LPS联合烟熏造模,通过病理、血气判断其COPD病理状态,记录造模完成后大鼠宏观表征变化并将数据定量化、四诊合参其证候归属。结果模型组大鼠平静及游泳后呼吸频率加快;体重减轻且增幅小;力竭游泳时间距离缩短、活动能力不足、四肢抓力下降;毛色亮度值偏低;舌色、爪色偏红、舌质暗红、舌下脉络迂曲的宏观表征符合气虚血瘀证主症、次症及舌脉的诊断标准。结论LPS联合烟熏可以稳定、重复、持续地造成大鼠气虚血瘀COPD证候模型。Objective Starting from quantitative analysis of rat macroscopic characterization,and taking"cause-pulsesyndrome-treatment"as the exploratory thinking,to analyze the syndrome attribution of the COPD rat model which was caused by LPS combined with smoking.Methods SD rats were randomly divided into two groups(blank group and model group).The model group was established by LPS combined with smoking.After modeling,the pathological state of COPD was judged by pathology and blood gas analysis.The macroscopic characteristics of the rats were observed and quantified,and comprehensive analysis syndrome type by using four diagnostic methods.Results The rats in the model group had accelerated breathing rate in a calm state or in swimming,lost weight and increased by small degrees.The time and distance was shortened in exhaustive swimming,activity ability was insufficient,the brightness value of coat color was low.Livid claw,dark purple tongue,sublingual varices,which was the macroscopic characteristics that accorded with the diagnostic criteria of main symptom,minor symptom and tongue condition of Qi deficiency and blood stasis syndrome.Conclusion LPS combined with smoking can steadily,repeatly,and continuously establish COPD rat model of Qi deficiency and blood stasis syndrome.

关 键 词：慢性阻塞性肺疾病 病证结合 宏观表征 气虚血瘀 

分 类 号：R256.1[医药卫生—中医内科学]