紫藤茎中的异黄酮类化学成分研究  

Study on isoflavones isolated from stems of Wisteria sinensis

作　　者：彭志茹 汪芝香 赵建平[2] 许琼明[1] 杨世林[1,3] PENG Zhi-ru;WANG Zhi-xiang;ZHAO Jian-ping;XU Qiong-ming;YANG Shi-lin(College of Pharmaceutical Scicnces,Soochow University,Suzhou 215123,China;National Center for Natural Products Research,University of Mississippi,Oxford 38677,America;College of Pharmaceutical Sciences,Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China)

机构地区：[1]苏州大学药学院,江苏苏州215123 [2]美国密西西比大学国家天然产物研究中心,密西西比牛津38677 [3]江西中医药大学药学院,江西南昌330006

出　　处：《中草药》2020年第15期3850-3855,共6页Chinese Traditional and Herbal Drugs

摘　　要：目的研究紫藤属植物紫藤Wisteria sinensis茎的化学成分。方法利用硅胶柱色谱、聚酰胺柱色谱、Sephadex LH-20凝胶柱色谱法及半制备高效液相色谱等方法分离纯化,并结合理化性质和现代波谱技术鉴定化合物结构。结果从紫藤茎甲醇提取物中分离得到14个异黄酮类化合物,分别鉴定为鸢尾黄素-7-O-β-D-(6″-O-乙酰基)-葡萄糖苷(1)、7,3′,4′-三羟基异黄酮(2)、芒柄花黄素(3)、阿佛洛莫生(4)、樱黄素(5)、鹰嘴豆芽素A(6)、7,3′,5′-三羟基-4′-甲氧基异黄酮(7)、8-甲雷杜辛(8)、鸢尾苷(9)、染料木苷(10)、降紫香苷(11)、芒柄花苷(12)、葛花宁(13)、苦参醇O(14)。结论化合物1是新化合物,命名为鸢尾苷A;化合物2、5、7、9~11、13、14为首次从紫藤属植物中分离得到。Objective To investigate the chemical constituents from the stems of Wisteria sinensis.Methods The 14 chemical constituents were isolated and purified by silica gel,polyamide,Sephadex LH-20 column chromatography,and semi-preparative HPLC,and their chemical structures were identified by physico-chemical constants and spectral data.Results Fourteen isoflavones were isolated from the methanol extract of the stems of W.sinensis and identified as tectorigenin-7-O-β-D-(6″-O-acetyl)-glucoside(1),7,3′,4′-trihydroxyisoflavanone(2),formononetin(3),afromosin(4),prunetin(5),biochanin A(6),gliricidin(7),8-O-methylreyusin(8),tectoridin(9),genistin(10),sissotrin(11),ononin(12),kakkanin(13),and kushenol O(14).Conclusion Compound 1 is a new compound named as tectoridin A,and compounds 2,5,7,9—11,13,and 14 are obtained from the genus Wisteria for the first time.

关 键 词：紫藤 异黄酮 鸢尾黄素-7-O-β-D-(6″-O-乙酰基)-葡萄糖苷 鸢尾苷A 樱黄素 葛花宁 苦参醇O 

分 类 号：R284.1[医药卫生—中药学]