旱生卷柏内生真菌Aspergillus ochraceus SX-C7次生代谢产物研究  

Secondary metabolites of endophytic fungus Aspergillus ochraceus SX-C7 from Selaginella stauntoniana

作　　者：罗萍[1] 邵刚 张升琪 朱丽 丁中涛[1] 蔡乐[1] LUO Ping;SHAO Gang;ZHANG Sheng-qi;ZHU Li;DING Zhong-tao;CAI Le(Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province,Key Laboratory of Medicinal Chemistry for Natural Resource,Ministry of Education,School of Chemical Science and Technology,Yunnan University,Kunming 650091,China)

机构地区：[1]云南大学云南省高校功能分子分析与生物转化重点实验室,教育部自然资源药物化学重点实验室,化学科学与工程学院,云南昆明650091

出　　处：《中草药》2020年第15期3856-3862,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81660719);长江学者和创新团队发展计划(IRT_17R94);云南省中青年学术和技术带头人后备人才(2018HB036);云南大学大学生创新实验项目(201804042)。

摘　　要：目的研究旱生卷柏Selaginella stauntoniana内生真菌赭曲霉菌(Aspergillus ochraceus SX-C7)的次生代谢产物。方法采用硅胶、SephadexLH-20、C-18反相柱色谱等进行分离和提纯,运用MS、NMR等波谱技术鉴定化合物的结构。采用二倍稀释法和Ellman法对分离得到的部分化合物进行抑菌活性和乙酰胆碱酯酶抑制活性测定。结果从赭曲霉菌(Aspergillus ochraceus SX-C7)的次生代谢产物中分离鉴定了10个化合物,分别为semivioxanthin(1)、6-hydroxy-p-menth-4(5)-en-3-one(2)、黄曲霉素(3)、木兰脂素(4)、preussinB(5)、circumdatinsD(6)、异岩藻甾醇(7)、sclerotiamide(8)、3β-hydroxyergosta-8,24(28)-dien-7-one(9)、3-O-β-D-glucopyranosyl stigmasta-5(6),24(28)-diene(10)。结论化合物1、2、4、7、10首次从赭曲霉菌中分离得到。活性研究表明化合物10对枯草芽孢杆菌具有很强的抑制活性,最小抑菌浓度(MIC)值为2μg/mL,抑制效果和阳性对照相当,化合物1对乙酰胆碱酯酶具有较强的抑制作用,抑制率为62.3%(终浓度为50μmol/L)。Objective To study the secondary metabolites of endophytic fungus Aspergillus ochraceus SX-C7 from Setaginella stauntoniana.Methods The compounds were isolated and purified by silica gel,Sephadex LH-20,C-18 reversed phase column chromatography,and their structures were identified by MS and NMR analyses.Antibacterial activity and inhibitory activity against acetylcholinesterase were studied by doubling dilution and Ellman methods.Results Ten known compounds were isolated from the secondary metabolites of A.ochrateus SX-C7,and identified as:semivioxanthin(1),6-hydroxy-p-menth-4(5)-en-3-one(2),flavacol(3),magnolin(4),preussin B(5),circumdatins D(6),isofucosterol(7),sclerotiamide(8),3β-hydroxyergosta-8,24(28)-dien-7-one(9),and 3-O-β-D-glucopyranosyl stigmasta-5(6),24(28)-diene(10).Conclusion Compounds 1,2,4,7,and 10 were isolated from A.ochraceus SX-C7 for the first time.The results of the bioactivity assay showed that compound 10 possessed obvious inhibitory activity against Bacillus subtilis with a MIC value of 2μg/mL,which was at the same grade with positive control.Compound 1 exhibited potent inhibitory activity against acetylcholinesterase with an inhibitory rate of 62.3%(the final concentration was 50μmol/L).

关 键 词：旱生卷柏 赭曲霉 次生代谢产物 抑菌活性 乙酰胆碱酯酶抑制活性 semivioxanthin 木兰脂素 异岩藻甾醇 

分 类 号：R284.1[医药卫生—中药学]