山腊梅叶提取物抗补体及镇咳活性物质基础的初步研究  

Primary research on components with anticomplement and antitussive activities from leave extracts of Chimonanthus nitens

作　　者：黄文平[1,2] 温芝琪 吴欢 王琦[1,2] 李志峰 周―斌[3] 李艳 冯育林[1,2] 杨世林 HUANG Wen-ping;WEN Zhi-qi;WU Huan;WANG Qi;LI Zhi-feng;ZHOU Bin;LI Yan;FENG Yu-lin;YANG Shi-lin(National Engineering Center of Solid Preparation of Chinese Materia Medical Manufacturing Technology,Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China;State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment,Nanchang 330006,China;The Pharmacy College of Jiangxi Science and Technology Normal University,Nanchang 330013,China)

机构地区：[1]江西中医药大学中药固体制剂制造技术国家工程研究中心,江西南昌330006 [2]创新药物与高效节能降耗制药设备国家重点实验室,江西南昌330006 [3]江西科技师范大学药学院,江西南昌330013

出　　处：《中草药》2020年第15期3869-3875,共7页Chinese Traditional and Herbal Drugs

基　　金：江西省杰出青年资助计划项目(20162BCB23003);江西省百千万人才工程资助[赣人社字(2016)332号];江西中医药大学学科建设项目(JXSYLXK-ZHYAO017);南昌市中药与天然药物活性成分研究知识创新团队(洪科字〔2018〕274号);江西省卫计委科研项目(2018B144)。

摘　　要：目的研究山腊梅Chimonanthus nitens叶提取物的抗补体及镇咳活性,并初步分析其活性成分。方法以经典抗补体途径和浓氨致咳模型对不同极性部位的山腊梅叶进行活性比较,确定具有抗补体和镇咳活性的部位;采用高效液相色谱-质谱联用技术对有效活性部位进行化学成分的初步分析。对山腊梅叶中主要的化学成分进行镇咳和抗补体的活性评价。结果山腊梅叶的醋酸乙酯提取物部位同时具有抗补体和镇咳作用,通过质谱分析,初步鉴定了28个化合物。山柰酚-3-O-芸香糖苷和山柰酚同时具有镇咳和抗补体的活性。结论山腊梅叶醋酸乙酯提取物具有良好的抗补体和镇咳活性,其主要活性成分为山柰酚-3-O-芸香糖苷和山柰酚,且两者可作为控制质量的化学标记物。Objective To investigate and analyze anticomplement and antitussive activities and the active ingredients of the extract of Chimonanthus nitens leaf.Methods The classical anti-complement pathway and the concentrated ammonia-induced cough model was used to compare the activity of the different polar parts of C.nitens leaf,and the polar parts with anti-complement and antitussive activity were determined.A preliminary analysis of the chemical composition in activity extract was identified by high performance liquid chromatography-mass spectrometry.The main chemical components in the C.nitens leaf of antitussive and antibody activity were also evaluated.Results The ethyl acetate extract of C.nitens leaf had both anti-complement and antitussive effects.A total of 28 compounds were initially identified through mass spectrometry analysis.Kaempferol-3-O-rutinoside and kaempferol had both antitussive and anti-complement activities.Conclusion The ethyl acetate extract of C.nitens leaf has good anti-complement and antitussive activities,and the mainly active ingredients in it were kaempferol-3-O-rutinoside and kaempferol that could be used as quality-controlling chemical markers.

关 键 词：山腊梅 抗补体 镇咳 高效液相色谱-质谱联用技术 山柰酚-3-O-芸香糖苷 山柰酚 

分 类 号：R284.1[医药卫生—中药学]