多基原兔耳草HPLC指纹图谱对比研究  

Comparation of HPLC fingerprints of Lagotis integra and Lagotis brevituba between different origins

作　　者：高必兴 苟琰 齐景梁 蒋桂华[1] 耿昭 周娟 GAO Bi-xing;GOU Yan;QI Jing-liang;JIANG Gui-hua;GENG Zhao;ZHOU Juan(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 610072,China;Sichuan Institute for Food and Drug Control/Key Laboratory of Quality Evaluation of Chinese Patent Medicines,NMPA,Chengdu 611731,China)

机构地区：[1]成都中医药大学药学院,四川成都610072 [2]四川省食品药品检验检测院/国家药品监督管理局中成药质量评价重点实验室,四川成都611731

出　　处：《中草药》2020年第15期4019-4024,共6页Chinese Traditional and Herbal Drugs

基　　金：国家食药监总局药化司专项“特色民族药材检验方法示范性研究”;国家重点研发计划子课题(2019YFC1712302);成都中医药大学杏林学者项目(XSGG2019023);成都中医药大学杏林学者项目(QNXZ2019024)。

摘　　要：目的建立现有国家药品标准收载的兔耳草基原全缘兔耳草Lagotis integra(药材名为兔耳草)及短管兔耳草L.brevituba(药材名为洪连)的HPLC指纹图谱方法,并通过所建立的方法对市场上其他3种较为常见的兔耳草(圆穗兔耳草L.ramalana、革叶兔耳草L.alutacea及短穗兔耳草L.brachystachya)进行对比研究。方法全缘兔耳草与短管兔耳草的HPLC指纹图谱相似度低,故分别建立二者HPLC指纹图谱方法;采用Waters X-Bridge C18(250 mm×4.6 mm,5μm);流动相为乙腈-0.2%甲酸水溶液,梯度洗脱;体积流量1.0 mL/min;检测波长328 nm;柱温35℃(全缘兔耳草)及25℃(短管兔耳草)。结果建立的全缘兔耳草HPLC对照指纹图谱指认了14个共有峰,并标定了大车前苷、鞭打绣球苷B、10-O-trans-p-methoxycinnamoyl-catalpol及10-O-[(E)-3,4-dimethoxycinnamoyl]catalpol峰;所建立的短管兔耳草HPLC对照指纹图谱存在13个共有峰,并标定松果菊苷、大车前苷及毛蕊花糖苷峰;建立的全缘兔耳草HPLC对照指纹图谱与所有批次全缘兔耳草及革叶兔耳草的相似度均大于0.9,与其他3种兔耳草相似度低;建立的短管兔耳草HPLC对照指纹图谱与所有批次短管兔耳草及圆穗兔耳草的相似度均大于0.9,与其他3种兔耳草相似度低。结论已建立的方法能有效地对各基原兔耳草进行鉴别,建议革叶兔耳草与全缘兔耳草同时收载于《卫生部药品标准(藏药第一册)》,圆穗兔耳草可作为《中国药典》2015年版一部收载的"洪连"的新基原。Objective To establish HPLC fingerprints of Lagotis integra and Lagotis brevituba which were contained in National Drug Standard and compared to Lagotis ramalana,Lagotis alutacea and Lagotis brachystachya by the established method.Methods The similarity of HPLC fingerprints of L.integra and L.brevituba was low,so the HPLC fingerprint methods were established for both.The HPLC analysis was performed on Waters X-Bridge C18(250 mm×4.6 mm,5μm),using acetonitrile and 0.2%formic acid solution as the mobile phase at a flow rate of 1.0 mL/min,the detection wave-length was 328 nm and column temperature was 35℃(L.integra)and 25℃(L.brevituba).Results There were 14 common peaks in the fingerprint of L.integra,and plantamajoside,hemiphroside B,10-O-trans-p-methoxycinnamoyl-catalpol and 10-O-[(E)-3,4-dimethoxycinna moyl]-catalpol were standardized.There were 13 common peaks in the fingerprint of L.brevituba,and echinacoside,plantamajoside and acteoside were standardized.The similarity of HPLC fingerprint was more than 0.9 between L.integra and L.alutacea,but the others had low similarity with them.The similarity of HPLC fingerprint was more than 0.9 between L.brevituba from different batches and L.ramalana,while the others had low similarity with them.Conclusion The established method could effectively identify L.brevituba,L.integra and L.alutacea were advised to be recorded in Medical Standards of the Ministry of Health.Lagotis ramalana could be used as a new base for"Honglian"(origin:Lagotis brevituba).

关 键 词：全缘兔耳草 短管兔耳草 圆穗兔耳草 革叶兔耳草 短穗兔耳草 HPLC指纹图谱 

分 类 号：R286.2[医药卫生—中药学]