紫杉醇类抗肿瘤原料药生产的研究进展  

Recent advances in synthesis of paclitaxel antitumor pharmaceutical raw materials

作　　者：熊亮斌 唐红菊 宋新巍 刘显舟 张馨文 计雨情 徐曼 徐一新[1] XIONG Liang-bin;TANG Hong-ju;SONG Xin-wei;LIU Xian-zhou;ZHANG Xin-wen;JI Yu-qing;XU Man;XU Yi-xin(Shanghai University of Medicine and Health Sciences,Shanghai 201318,China;Liming Research&Design Institute of Chemical Industry Co,Ltd.,Luoyang 471099,China)

机构地区：[1]上海健康医学院,上海201318 [2]黎明化工研究设计院有限责任公司,河南洛阳471099

出　　处：《中草药》2020年第15期4042-4049,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31370080);上海市教育委员会上海健康医学院医学技术Ⅱ类高峰高原学科建设计划(2018-2020);上海高校青年教师培养资助计划(ZZJKYX19011);上海健康医学院师资人才百人库。

摘　　要：紫杉醇类化合物属二萜类生物碱,是已知疗效最好的植物源抗肿瘤药物之一。目前,工业上主要依赖半化学合成、植物提取及细胞培养等途径大规模获取紫杉醇类产物。针对主流方法存在的问题,重点分析获取紫杉醇类活性产物的研发动向,探讨采用红豆杉树皮细胞培养、内生真菌发酵生产紫杉醇的技术现状与困境,简要梳理紫杉醇的天然合成途径,讨论采用合成生物学方法异源合成紫杉醇及其前体的可能性,为建立绿色、可持续的紫杉醇类活性产物生产路线提供参考。Paclitaxel,a diterpenoid alkaloid,is known as one of the most effective plant antitumor drugs.At present,paclitaxel products are mainly obtained by semi-chemical synthesis,extraction from Taxus spp.plant and bark cell culture synthesis.This study emphatically analyzed the research progress in the production of paclitaxel.The identification of genes related to paclitaxel biosynthesis was summarized.Furthermore,it was assessed that the possibility of using the bark cell culture of Taxus chinensis,endophytic fungi fermentation and the synthetic biology heterologous synthesis to produce paclitaxel compounds.The review can provide theoretical reference for the establishment of green and sustainable industrial production methods of the paclitaxel active products and their precursors.

关 键 词：紫杉醇 巴卡亭Ⅲ 合成 树皮细胞培养 内生真菌 合成生物学 

分 类 号：R284[医药卫生—中药学]