采用高效液相法测定甘草中多组分农药残留的方法研究  

Study on Determination of Multi-component Pesticide Residues in Licorice Root by HPLC

作　　者：汪盈盈 张黎娟[1,2] 王蓉 WANG Yingying;ZHANG Lijuan;WANG Rong(Bozhou Institute of Traditional Chinese Medicine,Anhui Academy of Chinese Medical Sciences,Bozhou 236800;School of Pharmacy,Bozhou Vocational and Technical College,Bozhou 236800)

机构地区：[1]安徽中医药科学院亳州中医药研究所,亳州236800 [2]亳州职业技术学院药学院,亳州236800

出　　处：《吉林农业科技学院学报》2020年第4期6-9,共4页Journal of Jilin Agricultural Science and Technology University

基　　金：安徽省高校自然科学研究重点项目(KJ2018A0883);安徽省高等学校省级质量工程项目(2017j×td082)。

摘　　要：该试验建立了甘草中4种氨基甲酸酯类农药多组分残留的液相色谱分析方法,选用Agilent 1260液相色谱,色谱柱:Acquity UPLCBEH C18柱(50 mm×2.1 mm,1.7 um)进行测定,在选定的实验条件下,4种氨基甲酯类农药的回收率在94.3%~98.1%,RSD在0.16%~0.34%。得出该方法灵敏度高、准确性好,符合农药多种残留检测的技术要求,适用于甘草提取物中氨基甲酸酯类农药残留的检测。In this paper,a liquid chromatography method for the determination of 4 carbamate pesticide residues in licorice was established.Agilent 1260 liquid chromatography was used for the determination with Acquity UPLC BEH C18 column(50 mm×2.1 mm,1.7 um).Under the selected experimental conditions,the recovery rate of 4 carbamate pesticides was 94.3%-98.1%,and the RSD was 0.16%-0.34%.The method has high sensitivity and accuracy,which meets the technical requirements of pesticide residue detection,and is suitable for the detection of carbamate pesticide residues in licorice extract.

关 键 词：高效液相色谱法 甘草 氨基甲酸酯 回收率 

分 类 号：O657.72[理学—分析化学] S481.8[理学—化学]