食品用塑料包装材料中双酚A和壬基苯酚的高效液相色谱-荧光法测定  

Determination of Bisphenol A and Nonyl Phenol in Plastic Materials for Food Packaging by High Performance Liquid Chromatography-Fluorescence Detector

作　　者：汪仕韬 纪丽君 龚珊 夏宝林 张维益 殷晶晶 姚卫蓉[3] WANG Shitao;JI Lijun;GONG Shan;XIA Baolin;ZHANG Weiyi;YIN Jingjing;YAO Weirong(Jiangyin Food Safety Testing Center,Jiangyin 214431;Suzhou Institute of Product Quality Supervision and Inspection,Suzhou 215104;School of Food Science and Technology,Jiangnan University,Wuxi 214422)

机构地区：[1]江阴市食品安全检测中心,江苏江阴214431 [2]苏州市产品质量监督检验院,江苏苏州215104 [3]江南大学食品学院,江苏无锡214122

出　　处：《分析科学学报》2020年第4期559-562,共4页Journal of Analytical Science

基　　金：国家重点研发计划(No.2018YFC1602300);江阴市社会发展科技示范项目(JY0603A020201190002PB)。

摘　　要：建立了食品用塑料包装材料中双酚A和壬基苯酚的高效液相色谱-荧光检测方法。考察了双酚A和壬基苯酚的最佳激发波长和发射波长、样品合适的萃取溶剂。样品以甲醇为萃取溶剂,温度50±5℃的条件下,超声60 min,浓缩后选用荧光激发波长225 nm,发射波长302 nm进行检测。双酚A和壬基苯酚在0.01~1.0 mg/L范围内线性良好,相关系数均大于0.999,方法检出限均为0.05 mg/kg。样品加标回收率在93.07%~107.54%之间,相对标准偏差(RSD)在1.9%~4.5%之间。所建立方法适用于食品用塑料包装材料中双酚A和壬基苯酚含量测定。A method for the determination of bisphenol A(BPA)and nonylphenol(NP)in plastic materials for food packaging by high performance liquid chromatography(HPLC)-fluorescence detection was established.The optimum excitation and emission wavelengths of BPA and NP as well as the appropriate extraction solvent were investigated.The samples were extracted with methanol at 50℃±5℃for 60 minutes,then concentrated and detected by HPLC-fluorescence detection with excitation wavelength of 225 nm and of emission wavelength of 302 nm.The calibration curve of BPA and NP showed good linear relationship in the range of 0.01 mg/L to 1.0 mg/L with correlation coefficients higher than 0.999.The detection limits of the method were 0.05 mg/kg for each compound.The recovery ranged from 93.07%to 107.54%,and the relative standard deviation(RSD)ranged from 1.9%to 4.5%.The results demonstrate this method is suitable for the determination of BPA and NP in plastic materials for food packaging.

关 键 词：食品包装 双酚A 壬基苯酚 高效液相色谱-荧光法 

分 类 号：O657.72[理学—分析化学]