辨证使用骨蚀宁胶囊Ⅰ号、Ⅱ号对激素性股骨头坏死家兔模型CD34、CYR61、CTGF表达影响的实验研究  

Experimental Study on the Effect of Gushining Capsules Ⅰ and Ⅱ on the Expression of CD34,CYR61 and CTGF in Rabbit model of Hormonal Femoral Head Necrosis

作　　者：朱磊[1] 周正新[1] 刘安平[1] 陈朝晖[2] 周导[1] 李文华[1] 康金平[1] ZHU Lei;ZHOU Zhengxin;LIU Anping;CHEN Zhaohui;ZHOU Dao;LI Wenhua;KANG Jinping(The First Affiliated Hospital of Anhui University of Chinese Medicine,Anhui Hefei 230031,China;Anhui University of Chinese Medicine,Anhui Hefei 230031,China)

机构地区：[1]安徽中医药大学第一附属医院,安徽合肥230031 [2]安徽中医药大学,安徽合肥230031

出　　处：《中医药临床杂志》2020年第8期1482-1486,共5页Clinical Journal of Traditional Chinese Medicine

基　　金：安徽中医药大学校级科研项目(2017fyzd004)。

摘　　要：目的:通过此研究,为骨蚀宁胶囊治疗激素性股骨头坏死提供数据依据。方法:选取60只新西兰大白兔,随机抽取6只不造模作为正常组,剩余54只造模成功后随机分为3组:骨蚀宁胶囊组(治疗组)、空白对照组(模型组)、健骨生组(阳性组),空白对照组灌胃生理盐水,阳性组灌胃健骨生丸,治疗组灌胃骨蚀宁Ⅰ号、Ⅱ号。分别于造模后第4周、8周、12周分批处死,每组6只,取股骨头标本切片,通过HE染色,观察股骨头组织形态变化,运用免疫组化法(SP二步法)观察股骨头CD34、CYR61及CTGF表达的情况。结果:模型组动物的一般状态差,骨坏死发生严重,治疗组动物一般状态较好,骨坏死发生轻,模型组空骨陷窝率明显高于治疗组,治疗组空骨陷窝率稍低于阳性组,组间比较差异有统计学意义。第4周:模型组、阳性组和治疗组的CD34、CYR61及CTGF灰度值均低于正常组,治疗组和阳性组的灰度值都低于模型组的灰度值,差异有统计学意义;第8周:治疗组和阳性组的灰度值此时仍低于模型组的灰度值,治疗组的灰度值与阳性组灰度值比较,治疗组偏低;第12周:正常组和阳性组的灰度值均高于治疗组,治疗组股骨头的CD34、CYR61及CTGF的表达高于正常组和阳性组的表达,差异有统计学意义。结论:骨蚀宁Ⅰ号、Ⅱ号均能够增强CD34、CYR61及CTGF表达,有利于坏死股骨头内血管重建与再生,使骨坏死区的血运得到改善,使骨坏死得到不同程度的修复;骨蚀宁胶囊通过改善局部血供、修复坏死骨组织,对激素性股骨头坏死起到一定的防治作用。Objective:Through this study,to provide data basis for the treatment of steroid-induced femoral head necrosis by Gushining capsule.Methods:Sixty New Zealand white rabbits were selected,and 6 rabbits were randomly selected as the normal group without modeling.The remaining 54 rabbits were randomly divided into 3 groups after successful modeling:Gushining capsule group(treatment group),blank control group(model group),Jian Gusheng group(positive group),the blank control group was gavage with normal saline,the positive group was gavage Jiangusheng pill,and the treatment group was gavage with Gushining No.Ⅰ and No.Ⅱ.They were sacrificed in batches at the 4th,8th,and 12th weeks after the model was created.There were 6 in each group.The femoral head specimens were sliced and stained with HE to observe the morphological changes of the femoral head.Using immunohistochemistry(SP two-step method)Observe the expression of CD34,CYR61 and CTGF in the femoral head.Results:The general state of the animals in the model group was poor,and the occurrence of osteonecrosis was severe.The animals in the treatment group were in good general state and the occurrence of osteonecrosis was mild.The empty bone lacuna rate in the model group was significantly higher than that in the treatment group,and the empty bone lacuna rate in the treatment group was slightly lower.In the positive group,the difference between the groups was statistically significant.Week 4:The gray values of CD34,CYR61 and CTGF of the model group,positive group and treatment group are all lower than the normal group,and the gray values of the treatment group and positive group are lower than the gray value of the model group,the difference is statistically significant Significance;8th week:the gray value of the treatment group and the positive group is still lower than the gray value of the model group at this time,the gray value of the treatment group is compared with the gray value of the positive group,the treatment group is lower;week 12:The gray values of the

关 键 词：骨蚀宁胶囊 激素性股骨头坏死 CD34 CYR61 CTGF 

分 类 号：R289.1[医药卫生—方剂学]