抗条锈病普通小麦-滨麦6BS·6NsS附加易位系的分子细胞遗传学鉴定  

Molecular Cytogenetic Identification of Wheat-Leymusmollis 6BS·6NsS Additional Translocation Line Conferring Stripe Rust Resistance

作　　者：杜少帅 林益达 于军伟 杜欣 杨晓莹 吉万全[1,2] 王长有 DU Shaoshuai;LIN Yida;YU Junwei;DU Xin;YANG Xiaoying;JI Wanquan;WANG Changyou(College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China;Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement,Ministry of Agriculture,Yanngling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]农业部作物基因资源与种质创制陕西科学观测实验站,陕西杨凌712100

出　　处：《麦类作物学报》2020年第8期897-905,共9页Journal of Triticeae Crops

基　　金：国家重点研究开发项目(2016YFD0102000);西北农林科技大学唐仲英育种基金项目。

摘　　要：易位系是向小麦转移外源种属优异基因的重要种质资源。普通小麦-滨麦衍生系M13063A-1由小麦-滨麦第六部分同源群二体异附加系材料连续自交获得。为了给普通小麦-滨麦衍生系M13063A-1的利用提供依据,本研究利用形态学、细胞学、原位杂交、分子标记等技术,对该材料进行了鉴定。细胞学鉴定结果显示,M13063A-1有丝分裂中期染色体数目为44条,减数分裂中期I染色体构型为2n=22Ⅱ,且在减数分裂后期Ⅰ可均等分离。原位杂交结果表明,M13063A-1含有42条普通小麦染色体和1对普通小麦-滨麦易位染色体,易位染色体长臂为滨麦Ns基因组染色体片段,短臂为6BS。分子标记分析确定M13063A-1携带的滨麦染色体片段为6NsS。成株期条锈病抗性鉴定显示,M13063A-1抗条锈菌生理小种条中31和条中32。因此,M13063A-1是一个抗条锈病的普通小麦-滨麦6BS·6NsS附加易位系,可以用作小麦抗病育种的桥梁材料。In order to provide the basis for the utilization of the common wheat-Leymusmollis derivative line M13063A-1,the material was identified by morphology,cytology,in situ hybridization and molecular markers.The cytological results showed that there were 44 chromosomes in M13063A-1 at metaphase of mitosis and 2n=22Ⅱat metaphase of meiosis,which could be equally separated at anaphase of meiosis.The results of in situ hybridization showed that M13063A-1 contained 42 chromosomes of common wheat and 1 pair of strand translocation chromosomes of common wheat-Leymusmollis.The long arm and short arm of translocation chromosome originated from Ns chromosomes of L.mollis and 6BS arm of wheat,respectively.Molecular marker analysis showed that M13063A-1 was 6Ns of L.mollis.The identification of resistance to stripe rust showed that M13063A-1 was resistant to Puccinia striiformisf.sp.tritici CYR31 and CYR32 at adult stage.Thus,the stripe rust-resistant wheat-L.mollis 6BS·6NsS additional translocation line M13063A-1 is a potentially useful germplasm for breeding disease-resistant wheat lines.

关 键 词：小麦 滨麦 附加易位系 原位杂交 分子标记 抗条锈病 

分 类 号：S512.1[农业科学—作物学] S330