非编码RNA GlmY对伤寒沙门菌生物膜形成的影响  

Effect of non-coding RNA GlmY on biofilm formation of Salmonella enterica serovar Typhi

作　　者：李雪 赵昕 靳梦彤 唐浩 张盈[1] 黄新祥[1] LI Xue;ZHAO Xin;JIN Meng-tong;TANG Hao;ZHANG Ying;HUANG Xin-xiang(School of Medicine,Jiangsu University,Zhenjiang Jiangsu 212013;Department of Nuclear Medicine,Nanjing First Hospital,Nanjing Jiangsu 210006,China)

机构地区：[1]江苏大学医学院,江苏镇江212013 [2]南京市第一医院核医学科,江苏南京210006

出　　处：《江苏大学学报（医学版）》2020年第5期435-438,共4页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(31670131)。

摘　　要：目的:探究非编码RNA GlmY对伤寒沙门菌生物膜形成的影响及其分子机制。方法:用自杀质粒pGMB151介导的同源重组方法,制备glmY缺陷株;用96孔微量板结晶紫染色法检测各菌株生物膜形成情况;通过qRT-PCR分析glmY缺陷株及野生株中生物膜形成相关基因的mRNA表达水平。结果:成功制备glmY缺陷株;细菌生物膜形成实验表明,与野生株相比,glmY缺陷株生物膜形成能力明显下降;qRT-PCR分析结果显示,GlmY缺失后卷曲菌毛合成相关基因csgD和csgA的mRNA表达水平明显下调。结论:伤寒沙门菌非编码RNA GlmY可通过上调csgD和csgA的表达促进卷曲菌毛合成,进而促进生物膜的形成。Objective:To investigate the effect of non-coding RNA GlmY on the biofilm formation of Salmonella enterica serovar Typhi(S.Typhi)and its potential molecular mechanisms.Methods:The homologous recombination induced by the suicide plasmid pGMB151 was used to generate the glmY deleted mutant of S.Typhi.The bacterial biofilm formation in 96-microwell plate was detected by crystal violet staining.qRT-PCR was used to analyze the mRNA expression levels of biofilm formation-related genes in g lmY deleted mutant and wild-type strain.Results:The glmY deletion mutant strain was successfully constructed.Compared with the wild-type strain,the glmY deletion mutant showed significantly weaker ability of biofilm formation.The qRT-PCR results showed that mRNA level of c sgD and csgA was significantly decreased after deletion of GlmY.Conclusion:The non-coding RNA GlmY may enhance the biofilm formation of S.Typhi by up-regulating the expression of csgD,csgA and Curli synthesis.

关 键 词：伤寒沙门菌 非编码RNA GlmY 生物膜 

分 类 号：R516.3[医药卫生—内科学]