骨康胶囊及其组分酢浆草及芭蕉根对大鼠软组织损伤的治疗作用  

Treatment of Soft Tissue Injury in Rats with Gukang Capsule,Oxalis Corniculata and Musa Basjoo Sieb

作　　者：李靖[1,2] 杨健 刘亭[1] 李勇军 沈艳珍[4] 杨畅[1] 何彬 王爱民[3] LI Jing;YANG Jian;LIU Ting;LI Yongjun;SHEN Yanzhen;YANG Chang;HE Bin;WANG Aimin(Guizhou Provincial Key Laboratory of Pharmaceutics&State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550004,Guizhou,China;School of Pharmacy,Guizhou Medical University,Guiyang 550025,Guizhou,China;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM of Ministry of Education,Guizhou Medical University,Guiyang 550004,Guizhou,China;Guizhou Weikang Zifan Pharmaceutical Co.,Ltd,Xiuwen 550200,Guizhou,China)

机构地区：[1]贵州医科大学,贵州省药物制剂重点实验室&省部共建药用植物功效与利用国家重点实验室,贵州贵阳550004 [2]贵州医科大学药学院,贵州贵阳550025 [3]贵州医科大学民族药与中药开发应用教育部工程研究中心,贵州贵阳550004 [4]贵州维康子帆药业股份有限公司,贵州修文550200

出　　处：《贵州医科大学学报》2020年第9期1015-1019,共5页Journal of Guizhou Medical University

基　　金：国家自然科学基金项目(U1812403);贵州省科技计划项目[黔科合J重大字(2015)2002、黔科合LH字(2016)7373];贵州省教育厅项目[黔教合重大专项字(2015)040];贵州省创新人才团队项目[黔科合平台人才(2016)5613、黔科合平台人才(2016)5677];中央引导地方科技专项项目[黔科中引地(2018)4006]。

摘　　要：目的:探讨骨康胶囊及其组分酢浆草及芭蕉根对大鼠软组织损伤的治疗作用。方法:80只大鼠随机均分为空白组、模型组、接骨七厘片组、骨康胶囊(低、中、高)剂量组、酢浆草组及芭蕉根组,后7组大鼠采用软组织损伤打击器(自制)制作左下肢局部急性软组织损伤模型,后6组大鼠分别灌胃给予相应药物7 d,空白组和模型组大鼠给予蒸馏水灌胃;灌胃结束后,测定大鼠血清组胺含量,取各组大鼠左下肢肌肉组织,HE染色观察组织形态学变化。结果:与模型组相比,接骨七厘片组、骨康胶囊(中、高)剂量组大鼠的血清组胺含量及肌肉组织形态学评分均降低,差异有统计学意义(P<0.05或P<0.01),酢浆草组、芭蕉根组大鼠血清组胺水平及组织学形态学评分变化不明显(P>0.05)。结论:骨康胶囊有利于大鼠软组织损伤修复,其作用机制可能与降低损伤局部组胺含量有关。Objective:To investigate the therapeutic effect of soft tissue injury in rats with Gukang capsule,oxalis corniculata and musa basjoo sieb.Methods:80 rats were randomly divided into blank group,model group,jieguqili tablet group,gukang capsule(low,medium and high)dose group,oxalis corniculata and musa basjoo sieb group.The model of local acute soft tissue injury of left lower extremity was made by soft tissue injury percussion device in 7 groups of rats.Each group of 6 rats was given with 7 days of corresponding drugs.Distilled water was given to rats in control and model groups.After gavage,the serum histamine content of rats was determined,the muscle tissue of the left lower extremity of each group was taken,and the histomorphological changes of HE staining were observed.Results:Compared with model group,serum histamine content and muscle histomorphology scores of rats in jieguqili tablet group and gukang capsule group were lower.The difference was statistically significant(P<0.05 or P<0.01).There was no significant change in serum histamine level and histology score of rats in oxalis corniculata and musa basjoo sieb group(P>0.05).Conclusion:Gukang capsule is beneficial to the repair of soft tissue injury in rats,and its mechanism may be related to the reduction of histamine content in the injured area.

关 键 词：软组织损伤 组胺 大鼠 骨康胶囊 酢浆草 芭蕉根 组织形态学 

分 类 号：R965[医药卫生—药理学]