miR-10b对胃癌细胞增殖和凋亡的影响及机制  

Effect of microRNA-10b on Proliferation and Apoptosis of Gastric Cancer Cells and its Mechanism

作　　者：张雪莲[1] 吴维宇[1] 何峰[1] ZHANG Xuelian;WU Weiyu;HU Feng(The First Affiliated Hospital of Chengdu Medical College,Department of Gastroenterology,Chengdu 610500,Sichuan,China)

机构地区：[1]成都医学院第一附属医院消化内科,四川成都610500

出　　处：《贵州医科大学学报》2020年第9期1056-1060,1071,共6页Journal of Guizhou Medical University

基　　金：四川省卫计委项目(17PJ013)。

摘　　要：目的:探索miR-10b在胃癌细胞中的表达及对胃癌细胞增殖和凋亡的影响及相关机制。方法:选取20例确诊胃癌患者,取胃癌组织标本及其癌旁正常组织标本,采用实时荧光定量PCR(qRT-PCR)检测miR-10b和上皮型钙黏蛋白(E-cadherin);利用Lip2000转染试剂盒说明书,将miR-10b转染至对数生长期的人胃癌细胞系,miR-NC作为空白对照组,qRT-PCR检测胃癌组织和细胞中miR-10b以及E-cadherin表达的变化;Western blot检测胃癌组织和细胞中E-cadherin和凋亡蛋白B淋巴细胞瘤-2基因(Bcl2)、Bcl2相关X蛋白(Bax)表达变化;噻唑蓝(MTT)法检测MKN-45细胞增殖能力的改变。结果:qRT-PCR结果显示,与癌旁组织比较,胃癌组织中miR-10b的表达升高,而E-cadherin的表达降低(P<0.05);与空白对照组比较,低分化胃癌组织和MKN-45癌细胞miR-10b表达增高,E-cadherin表达降低,差异有统计学意义(P<0.05);与空白对照组相比,转染miR-10b siRNA后,E-cadherin和Bax表达增加,Bcl2表达和细胞生长增殖抑制率降低,差异均有统计学意义(P<0.05);与空白对照组相比,GES-1细胞转染miR-10b mimics后,细胞内E-cadherin和Bax表达下降,Bcl2表达和细胞生长增殖抑制率增加,差异均有统计学意义(P<0.05)。结论:miR-10b能促进胃癌细胞增殖并抑制癌细胞的凋亡,其机制可能与调控E-cadherin有关。Objective:To explore the expression of miR-10b in gastric cancer cells and its effect on the proliferation and apoptosis of gastric cancer cells and its possible mechanism.Methods:20 patients with gastric cancer were enrolled in this study.Gastric cancer tissue samples and matched normal tissue samples were collected.MiR-10b and E-cadherin were detected by quantitative real-time PCR(qRT-PCR).The miR-NC(negative control)and miR-10b were transfected into human gastric cancer cell line with logarithmic phase using the method provided by Invitrogen Lip2000 Transfection Kit.The qRT-PCR was used to detect the expression changes of gastric cancer cellular tissues and miR-10b and E-cadherin.Western Blot was used to detect the expression changes of E-cadherin and apoptosis proteins Bcl2 and Bax in gastric cancer tissues and cells.MTT assay was used to detect the proliferation of MKN-45 cells.Results:The results of qRT-PCR showed that the expression of miR-10b in gastric cancer tissues was significantly higher than that of the adjacent tissues,while the expression of E-cadherin significantly decreased(P<0.05).Compared with the control group,the expression of miR-10b increased and the expression of E-cadherin decreased in poorly differentiated gastric cancer cells(P<0.05).The expression of E-cadherin increased after transfection of miR-10b siRNA,and the expression of Bcl2 markedly decreased;the expression of Bax increased,and the cell proliferation inhibition rates significantly decreased as compared with the control group(P<0.05).After transfection of miR-10b mimics in GES-1 cells,the expression of E-cadherin obviously decreased,the expression of Bcl2 effectively increased,and the expression of Bax effectively decreased.The cell proliferation inhibition rates obviously increased,and the difference was statistically significant compared with the control group(P<0.05).Conclusion:MiR-10b can promote the proliferation and suppress the cell apoptosis of gastric cancer cells,and its mechanism may be related to the regulation of

关 键 词：胃肿瘤 细胞增殖 细胞凋亡 微小RNA10b 上皮型钙黏蛋白 

分 类 号：R34[医药卫生—基础医学]