不同侵染时间点稻粒黑粉病菌的转录组分析  

Transcriptome analysis of Tilletia horrida at different infection time points

作　　者：舒新月 江波[3] 马丽 郑爱萍[1,2] SHU Xin-yue;JIANG Bo;MA Li;ZHENG Ai-ping(Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China;Key Laboratory of South-west Crop Gene Research & Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya’an 625014, China;College of Life Science and Technology, Yangtz Normal University, Chongqing 408100, China)

机构地区：[1]四川农业大学水稻研究所,四川温江611130 [2]四川农业大学西南作物基因资源与遗传改良教育部重点实验室,四川雅安625014 [3]长江师范学院生命科学与技术学院,重庆408100

出　　处：《草业学报》2020年第9期190-202,共13页Acta Prataculturae Sinica

基　　金：重庆市自然科学基金(cstc2018jcyjAX0594);涪陵区科委项目(FLKW,2017ABB1029)资助。

摘　　要：由土传真菌稻粒黑粉菌引起的稻粒黑粉病是水稻杂交制种田中的主要病害之一。为初步解析稻粒黑粉病菌侵染过程中与寄主的互作分子机制,本研究用稻粒黑粉病菌强毒力菌株JY-521侵染感病不育系材料“9311A”,于侵染8、12、24、48和72 h 5个时间点取菌样进行转录组测序分析。结果表明,与未侵染对照组相比,在侵染8 h时,差异表达基因(DEGs)数最多,有337个DEGs;其中上调的DEGs有120个,下调的DEGs有217个。337个DEGs的KEGG富集分析表明,脂肪酸代谢和过氧化物酶体可能是稻粒黑粉病菌侵染过程中的关键代谢途径。此外,对编码碳水化合物酶、分泌蛋白和宿主植物—病原互作蛋白的DEGs在5个侵染时间点的表达模式进行解析,结果表明,一些显著上调表达的DEGs可能是稻粒黑粉病菌侵染的关键致病因子。该研究结果为解析稻粒黑粉病菌的致病分子机制,进一步防控该病害奠定了理论基础。Rice kernel smut(RSB)caused by Tilletia horrida,is one of the most important rice diseases in hybrid rice growing areas worldwide.In order to classify the mechanisms of pathogenicity,transcriptome analysis of the T.horrid strain JY-521 was conducted at different times post inoculation(8,12,24,48,and 72 h)early in the infection.The highest number of differentially expressed genes(DEGs)occurred at 8 h post inoculation.Based on kyoto encyclopedia of genes and genomes(KEGG)pathway analysis of the DEGs,autophagy processes and lipid degradation were key pathways for T.horrida pathogenicity.The expression patterns of carbohydrate-active enzyme genes,pathogen-host interaction genes,and secreted protein genes were also analyzed at different times during the infection,and some DEGs that may play an important role in pathogenic progress of T.horrid were found.In summary,this research provides a new foundation for future study of the infection mechanism and control of this important rice disease.

关 键 词：水稻 稻粒黑粉病 转录组 致病机制 

分 类 号：S43[农业科学—农业昆虫与害虫防治]