紫毛野牡丹提取物抑真菌活性研究  

Antibacterial activity of the water extract of Melastoma genicillatum Naud

作　　者：王智宇 刘东 刘侠[1] 杨卫丽[1] WANG Zhi-yu;LIU Dong;LIU Xia;YANG Wei-li(School of Pharmacy,Hainan Medical University,Haikou 571199,Hainan,CHINA)

机构地区：[1]海南医学院药学院,海南海口571199

出　　处：《海南医学》2020年第17期2181-2183,共3页Hainan Medical Journal

基　　金：海南省自然科学基金(编号:20158282);海南省大学生创新创业训练计划项目(编号:2017115);海南医学院药学院创新实验计划项目(编号:HYCX2016072)。

摘　　要：目的研究紫毛野牡丹提取物的体外抑制真菌的作用。方法采用纸片法与微量稀释法分别考察紫毛野牡丹水提物及70%乙醇提取物对白假丝酵母菌(Ca)、热带假丝酵母菌(Ck)、光滑假丝酵母菌(Ct)、克柔假丝酵母菌(Cp)及近平滑假丝酵母菌(Cg)的体外抑制作用及相应最低抑菌浓度(MIC)。结果纸片法中水提物对Ca、Ck、Ct、Cp、Cg的平均抑菌圈直径分别为8.6 mm、9.0 mm、9.9 mm、9.7 mm、10.3 mm,70%乙醇提取物对Ca、Ck、Ct、Cp、Cg的平均抑菌圈直径分别为13.0 mm、11.4 mm、18.8 mm、16.1 mm、13.1 mm;微量稀释法试验中显示紫毛野牡丹水提取物对Ca的MIC为32μg/mL,对Ck的MIC为256μg/mL,对Cg的MIC为32μg/mL,对Ct的MIC为128μg/mL,对Cp的MIC为128μg/mL,70%乙醇提取物对Ca的MIC为32μg/mL,对Ck的MIC为128μg/mL,对Cg的MIC为32μg/mL,对Ct的MIC为64μg/mL,对Cp的MIC为128μg/mL。结论紫毛野牡丹水提取物及70%乙醇提取物对五种假丝酵母菌具有抑菌作用。Objective To investigate the antibacterial activity of the water extract of Melastoma genicillatum Naud.Methods The antifungal activity of the water extract and 70%ethanol extract against five Candidas(including C.albicans,C.tropical,C.glabrata,C.krusei,C.parapsilosis)was investigated employing the filter paper disc diffusion and micro-dilution.Results In the disc diffusion method,the average diameters of inhibition zone of water extract against Ca,Ck,Ct,Cp,and Cg were 8.6 mm,9.0 mm,9.9 mm,9.7 mm,10.3 mm,and that of 70%ethanol extract were 13.0 mm,11.4 mm,18.8 mm,16.1 mm,13.1 mm.In addition,the minimal inhibitory concentration(MIC)of the water extract against Ca,Ck,Cg,Ct,and Cp was 32μg/mL,256μg/mL,32μg/mL,128μg/mL,and 128μg/mL,respectively,while the MIC of the 70%ethanol extract against the same fungi was 32μg/mL,128μg/mL,32μg/mL,64μg/mL,and 128μg/mL,respectively.Conclusion The antifungal activity of the water extract of Melastoma genicillatum Naud and 70%ethanol extract were significant in the micro dilution method.

关 键 词：紫毛野牡丹 抑菌作用 白假丝酵母菌 热带假丝酵母菌 光滑假丝酵母菌 克柔假丝酵母菌 近平滑假丝酵母菌 

分 类 号：R93[医药卫生—生药学]