外周血pNF-H、S100B水平与脊柱骨折伴脊髓损伤患者病情程度的相关性  

Correlation between the expression of pNF-H and S100B in peripheral blood and the severity of spinal fracture complicated with spinal cord injury

作　　者：王晓林[1] 曾凡伟[1] Wang Xiaolin;Zeng Fanwei(Department of Orthopedics,Dazhou Central Hospital,Dazhou 635000,China)

机构地区：[1]四川达州市中心医院骨科,达州635000

出　　处：《新医学》2020年第9期710-713,共4页Journal of New Medicine

摘　　要：目的探讨外周血神经丝蛋白H磷酸化亚型(pNF-H)、S100B钙结合蛋白(S100B)水平与脊柱骨折伴脊髓损伤患者病情程度的相关性。方法选择238例脊柱骨折患者,根据其是否伴脊髓损伤分为脊髓损伤组71例与脊髓未损伤组167例,采用ELISA检测血清pNF-H、S100B水平,比较2组血清pNF-H、S100B水平,对比病情不同严重程度的脊柱骨折伴脊髓损伤患者血清pNF-H、S100B水平,绘制受试者工作特征(ROC)曲线分析血清pNF-H、S100B水平对脊柱骨折伴脊髓损伤的诊断价值。结果脊髓损伤组血清pNF-H、S100B水平高于脊髓未损伤组(P均<0.05)。脊髓完全损伤的脊柱骨折患者血清pNF-H、S100B水平高于脊髓不完全损伤的脊柱骨折患者(P均<0.05)。血清pNF-H、S100B水平联合检测对脊柱骨折伴脊髓损伤的ROC曲线下面积均高于单独检测(P均<0.05)。结论pNF-H、S100B水平在脊柱骨折伴脊髓损伤患者外周血中高表达,并与脊柱骨折伴脊髓损伤患者病情严重程度相关,检测血清pNF-H、S100B水平有助于脊柱骨折伴脊髓损伤早期诊断及病情严重程度评估。Objective To investigate the correlation between the expression levels of phosphorylated heavy-chain neurofilament(pNF-H)and S100B in the peripheral blood and the severity of spinal fracture complicated with spinal cord injury.Methods A total of 238 patients with spinal fractures were enrolled and divided into the spinal cord injury group(n=71)and non-spinal cord injury group(n=167)according to whether they were complicated with spinal cord injury.The serum pNF-H and S100B levels were quantitatively measured by enzyme-linked immunosorbent assay(ELISA)and statistically compared between two groups.In addition,the serum pNF-H and S100B levels were statistically compared among patients with varying severity of diseases.The receiver operating characteristic(ROC)curve was delineated to evaluate the diagnotic values of serum pNF-H and S100B levels for spinal fracture complicated with spinal cord injury.Results Serum pNF-H and S100B levels in the spinal cord injury group were significantly higher than those in the non-spinal cord injury group(both P<0.05).Serum pNF-H and S100B levels in patients with complete spinal cord injury were significantly higher than those in their counterparts with incomplete spinal cord injury(both P<0.05).The area under ROC curve(AUC)of combined detection of serum pNF-H and S100B for spinal fracture complicated with spinal cord injury was significantly higher than that of single detection(both P<0.05).Conclusions Both pNF-H and S100B are highly expressed in the peripheral blood of patients with spinal fracture complicated with spinal cord injury,which are significantly correlated with the severity of the diseases.The detection of two biomarkers contributes to early diagnosis and severity assessment of spinal fracture complicated with spinal cord injury.

关 键 词：神经丝蛋白H磷酸化亚型 S100B钙结合蛋白 脊柱骨折 脊髓损伤 表达水平 病情严重程度 相关性 

分 类 号：R651.2[医药卫生—外科学] R683.2[医药卫生—临床医学]