黑龙江省部分有色稻种质资源的遗传多样性分析  

Genetic Diversity Analysis of Some Colored Rice Germplasms in Heilongjiang Province

作　　者：徐丽丽 刘书涵 李世伟 康绍玲 王敬国[1] 付俊生 韩笑 孙健[1] XU Lili;LIU Shuhan;LI Shiwei;KANG Shaoling;WANG Jingguo;FU Junsheng;HAN Xiao;SUN Jian(College of Agriculture,Northeast Agricultural University,Haerbin Heilongjiang 150030,China)

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《种子》2020年第8期34-39,共6页Seed

基　　金：东北农业大学大学生创新创业项目(201910224032);黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2017024);国家自然科学基金(31701507);东北农业大学“学术骨干”项目(18 XG 02);黑龙江省博士后科研启动金(LBH-Q 17016)。

摘　　要：为阐明黑龙江省有色稻资源的遗传基础,为有色稻资源的有效利用及育种的亲本组配提供参考,利用58对SSR(Simple Sequence Repeats)引物对黑龙江省41份有色水稻资源和1份云南有色籼稻资源进行遗传多样性分析。共检测出等位基因224个,平均每对引物检测出3.86个。SSR位点的遗传多态性信息含量(PIC,polymorphic information content)在0.0454~0.3744之间,平均值为0.1443,基因多样性值为0.1617。不加权成对群算术平均数方法(UPGMA,Unweight pair group method using arithmetic averages)聚类分析结果显示,所选用的42个有色稻米品种的遗传相似性系数在0.21处可以分为两大类,在0.53处可以分为三大类,供试品种间相似系数大部分在0.70以上,最高可达0.95。结果表明,选用的41个黑龙江省有色稻米品种总体的遗传多样性差异较小,遗传背景大体相似,说明黑龙江有色稻米的遗传基础较为狭窄,在育种工作中应该注意加强资源创新,扩大黑龙江省有色稻米的种质资源库。In order to clarify the genetic basis of colored rice germplasm resources in Heilongjiang province and provide reference for the effective utilization of colored rice resources and the parental combination of breeding,genetic diversity analysis was conducted by using 58 simple sequence repeats(SSR)primers for 41 coloured rice resources from Heilongjiang province and 1 coloured indica rice resource from Yunnan province.The results showed that a total of 224 alleles were detected and an average of 3.86 were detected per pair of primers.The polymorphic information content(PIC)of SSR loci was in between 0.0454-0.3744,with a mean value of 0.1443 and genetic diversity value of 0.1617.Further,Cluster analysis based on unweight pair group method using arithmetic averages(UPGMA)showed that the selected 42 colored rice varieties could be divided into two categories at the genetic similarity coefficient being 0.21 while these rice varieties could be divided into three categories at the genetic similarity coefficient being 0.53.The similarity coefficient between the most of tested varieties was above 0.70 and up to 0.95.In brief,the results showed that the genetic diversity of the 41 selected colored rice varieties in Heilongjiang province was small and the genetic background was similar,indicating that the genetic basis of colored rice in Heilongjiang province was relatively narrow,so attention should be paid to strengthening resource innovation in breeding and expanding the germplasm resource pool of colored rice in Heilongjiang province.

关 键 词：黑龙江 有色稻 种质资源 SSR标记 遗传多样性分析 

分 类 号：S511[农业科学—作物学]