高效液相色谱法同时测定补肾清热合剂中哈巴苷、芍药苷和芒果苷的含量  

Simultaneous determination of harpagide,paeoniflorin and mangiferin in Bushen Qingre mixture by HPLC

作　　者：熊友健[1] 胡榕[1] 卢庆红[1] 涂琼[1] 陈梦莹 雷光远[1] XIONG You-jian;HU Rong;LU Qing-hong;TU Qiong;CHEN Meng-ying;LEI Guang-yuan(Jiangxi Provincial Children’s Hospital,Nanchang 330006)

机构地区：[1]江西省儿童医院,南昌330006

出　　处：《中南药学》2020年第8期1377-1380,共4页Central South Pharmacy

基　　金：江西省卫生计生委中医药科技计划项目(编号:2015A064)。

摘　　要：目的建立HPLC法同时测定补肾清热合剂中哈巴苷、芍药苷和芒果苷的含量。方法采用Kromasil C18柱(250 mm×4.6 mm,5 μm),流动相乙腈-0.2%磷酸水=15∶85(v/v),流速1.0 mL·min-1,柱温30℃,检测波长230 nm。结果哈巴苷、芍药苷和芒果苷分别在3.86~246.84 μg·mL-1(r=0.9998)、15.47~990 μg·mL-1(r=0.9999)和2.89~185 μg·mL-1(r=0.9997)与峰面积线性关系良好,平均加样回收率分别在99.2%~101.6%、100.1%~101.9%和99.1%~101.5%,RSD均<3.4%(n=3)。结论本法简便、专属性强、灵敏度高、结果准确可控,可用于补肾清热合剂的质量控制。Objective To simultaneously determine harpagide,paeoniflorin and mangiferin in Bushen Qingre mixture by HPLC.Methods The Kromasil C18 column (250 mm×4.6 mm,5 μm) was used.Acetonitrile-0.2% phosphoric acid solution (15∶85) (v/v) was used as the mobile phase at a flow rate of 1.0 mL·min-1.The wavelength was 230 nm,and the column temperature was 30℃.Results The linearity was 3.86~246.84 μg·mL-1 (r=0.9998) for harpagide,15.47~990 μg·mL-1 (r=0.9999) for paeoniflorin,and 2.89~185 μg·mL-1 (r=0.9997) for mangiferin.The recovery was 99.2%~101.6%,100.1%~101.9% and 99.1%~101.5%,respectively.RSDs were less than 3.4% (n=3).Conclusion The method is simple,specific,highly sensitive and accurate,which is suitable for the quality control of Bushen Qingre mixture.

关 键 词：补肾清热合剂 哈巴苷 芍药苷 芒果苷 

分 类 号：R917[医药卫生—药物分析学]