伽马氨基丁酸浸种对铝胁迫下白三叶种子萌发及耐铝性的影响  

Effects of seeds soaking withγ-aminobutyric acid on germination and aluminium tolerance of white clover

作　　者：符京燕 梁林林 周敏 李州[1] FU Jing-yan;LIANG Lin-lin;ZHOU Min;LI Zhou(College of Animal Science and Technology,Sichuan Agricultural University,Chengdu,Sichuan Province 611130,China)

机构地区：[1]四川农业大学动物科技学院草学系,四川成都611130

出　　处：《草地学报》2020年第5期1275-1284,共10页Acta Agrestia Sinica

基　　金：国家大学生创新创业训练计划项目(201810626007)资助。

摘　　要：本研究以‘海发’白三叶(Trifolium repens‘Haifa’)为供试材料,研究伽马氨基丁酸(γ-aminobutyric acid,GABA)浸种预处理对铝(aluminium,Al^3+)胁迫下白三叶种子萌发及耐Al^3+性的影响。研究表明,1 mmol·L^-1的Al^3+溶液即能显著抑制白三叶种子的萌发,随着Al^3+浓度的加大这种抑制作用也越明显。GABA浸种预处理显著提高Al^3+胁迫下白三叶种子的萌发和根长,且在4 mmol·L^-1的Al^3+胁迫下效果最明显。GABA浸种也显著提高Al^3+胁迫下抗氧化酶活性及非酶抗氧化物质含量,从而有效降低Al^3+胁迫导致的氧化伤害,提高细胞膜稳定性。此外,GABA浸种也进一步上调了Al^3+胁迫诱导的脱水蛋白基因表达。因此,GABA提高Al^3+胁迫下白三叶种子的萌发可能与增强抗氧化防御以减轻胁迫造成的氧化损伤及上调脱水蛋白基因表达有关。White clover(Trifolium repens‘Haifa’)was selected as experimental material in this study,and effects of seeds soaking with gamma-aminobutyric acid(GABA)on germination and tolerance to aluminium(Al^3+)stress were studied.The results showed that 1 mmol·L^-1 Al^3+solution significantly inhibited seeds germination,and the damage becomes more obvious with the increase of Al^3+concentration.The germination and root length of seeds soaking with GABA significantly increased under Al^3+stress with the most obvious effect under 4 mmol·L^-1 Al^3+stress.The antioxidant enzyme activity and non-enzymatic antioxidants content of seeds soaking with GABA significantly increased as well,thereby reducing oxidative damage effectively and improving cell membrane stability during germination under Al^3+stress.In addition,seeds soaking with GABA also significantly increased genes expression of dehydrins under Al^3+stress.Therefore,GABA-induced Al^3+tolerance could be related to the enhancement of antioxidant defense to reduce oxidative damage caused by stress and the up-regulation of dehydrins genes expression during white clover seeds germination.

关 键 词：非生物胁迫 抗氧化 氧化伤害 脱水蛋白 抗坏血酸-谷胱甘肽循环 

分 类 号：Q945.78[生物学—植物学]