高中生物“探究酵母菌种群数量的变化”实验方案改进  

Improvement on the Senior School Biological Experiment of Changing of Yeast Population Quantity in the Wine

作　　者：杜静[1] 吕乐[2] DU Jing;LV Le(Yuxin School Affiliated to Capital Normal University,BeiJing 100096;School of Chemical and Biological Engineering,University of Science and Technology Beijing,Beijing 100083)

机构地区：[1]首都师范大学附属育新学校,北京100096 [2]北京科技大学化学与生物工程学院,北京100083

出　　处：《科教导刊》2020年第21期150-151,160,共3页The Guide Of Science & Education

基　　金：北京市教育科学规划2017年度一般课题,课题名称为“基于生物科学素养培养的高中生物研究型实验探究”(编号CDDB17204)的研究成果。

摘　　要：酵母菌的种群数量增长实验是高中生物教学中的一个综合性探究实验,是课程标准要求中的重要部分。本文对课程要求实验方法进行丰富和改进,以自制果酒分离获得的酵母菌株为实验材料,进行18S rDNA分子鉴定。在预实验的基础上,对酵母菌进行液体培养,利用分光光度计测定种群数量动态变化并绘制其生长曲线,分析酿酒酵母的生长特性及影响因素。The population growth experiment of Saccharomyces cerevisiae is a comprehensive exploration experiment in biology teaching of senior school.It is an important part of the curriculum standard.In this paper,the experimental methods required by the course were enriched and improved.Yeast strains isolated from fruit wine were used as experimental materials for 18S rDNA molecular identification.On the basis of the pre-experiment,the yeast was cultured in liquid medium,the population dynamic change was measured by spectrophotometer and its growth curve was drawn,and the growth characteristics and influencing factors of Saccharomyces cerevisiae were analyzed.

关 键 词：酿酒酵母 种群数量 生长曲线 

分 类 号：G424[文化科学—课程与教学论]