贵州小麦品种(系)中慢锈基因Lr34/Yr18的分子检测  

Molecular Detection of Slow Rust Gene Lr34/Yr18 inGuizhou Wheat Varieties

作　　者：杨雪敏 任明见[1,2] 李鲁华 徐如宏[1,2] YANG Xuemin;REN Mingjian;LI Luhua;XU Ruhong(College of Agriculture,Guizhou University,Guiyang 550025,China;Guizhou Branch of National Wheat Improvement Center,Guiyang 550025,China)

机构地区：[1]贵州大学农学院,贵阳550025 [2]国家小麦改良中心贵州分中心,贵阳550025

出　　处：《种子》2020年第7期37-42,共6页Seed

基　　金：国家自然科学基金(31660390);国家“七大农作物育种”重点专项(2017 YFD 0100900);贵州省农业成果转化计划(黔科合成果[2016]4022号);贵州省科技计划项目(黔科合基础[2019]1073号)。

摘　　要：小麦慢锈病是危害小麦生产的重要病害。为了鉴定258份贵州小麦品种(系)中慢锈基因Lr34/Yr18的组成,筛选含慢锈抗性基因Lr34/Yr18的种质资源,本研究利用STS标记csLV 34结合毛细管电泳技术对258份小麦品种(系)中慢锈抗性基因Lr34/Yr18的等位变异进行了分子检测。结果表明:毛细管电泳谱带清晰易读,可根据扩增片段分子量直接判断目标片段有无。STS标记csLV 34可在含有Lr34/Yr18基因的材料中扩增出150 bp片段,部分不含Lr34/Yrl8的材料则扩增出229 bp片段,余下大部分不含Lr34/Yrl8的材料没有扩增出150 bp和229 bp的片段;258份小麦品种(系)中有5份材料扩增出150 bp片段,可能含有Lr34/Yr18基因,占供试材料的1.9%。筛选的这些慢锈抗性种质资源可为今后贵州小麦的慢锈抗病品种选育提供参考。The results showed that the capillary electrophoresis band was clear and easy to read,and the target fragment could be directly judged based on the molecular weight of the amplified fragment.The STS marker csLV 34 could amplify a 150 bp fragment in materials containing the Lr34/Yr18 gene and part of materials without Lr34/Yrl8 could amplify a 229 bp fragment while most of the remaining materials without Lr34/Yrl8 could not amplify either 150 bp or 229 bp fragment.Among the 258 wheat varieties(lines),5 materials might that might contain Lr34/Yr18 gene amplified 150 bp fragments,accounting for 1.9%of all the test materials.Finally,the selected resources of slow rust resistance germplasm can provide reference for the breeding of slow rust resistance wheat in Guizhou in the future.

关 键 词：小麦 慢锈基因 STS标记 毛细管电泳 

分 类 号：S512.1[农业科学—作物学]