四川彭州大蒜根腐病发病土壤细菌与真菌群落结构  

The community structure of bacteria and fungi in soils with root rot diseased garlic plants in Pengzhou,Sichuan Province

作　　者：余金阳 黄潇慧 帅正彬[2] 解亮 刘轶豪 王超仁 柴丹 刘蝶 周晓宇 陈强 YU Jinyang;HUANG Xiaohui;SHUAI Zhengbin;XIE Liang;LIU Yihao;WANG Chaoren;CHAI Dan;LIU Die;ZHOU Xiaoyu;CHEN Qiang(College of Resources,Sichuan Agricultural University,Chengdu 611130,China;Institute of Horticulture,Chengdu Academy of Agriculture and Forestry Sciences,Chengdu 611130,China)

机构地区：[1]四川农业大学资源学院,成都611130 [2]成都市农林科学院园艺研究所,成都611130

出　　处：《应用与环境生物学报》2020年第4期928-935,共8页Chinese Journal of Applied and Environmental Biology

基　　金：四川省重点研发项目(2019YFN0149);四川农业大学学科建设双支计划资助。

摘　　要：为了解土壤微生物群落与大蒜根腐病之间的关系,采集四川省彭州市不同地点大蒜正常生长和发病土壤样品,采用Illumina MiSeq测序技术分析土壤细菌与真菌的群落结构变化.结果显示,供试土壤的优势细菌主要为变形菌门和绿弯菌门,优势真菌主要为子囊菌门和担子菌门;与正常土壤相比,发病土壤细菌多样性减少,真菌多样性和丰富度增加,其中硝化螺菌属、Nitrosomonadaceae-uncultured、芽孢杆菌属和鞘氨醇单胞菌属等有益细菌及青霉属、木霉属和热霉属等有益真菌丰度降低,匐柄霉属和镰刀菌属等病原真菌丰度增加.Spearman分析结果显示,匐柄霉属和镰刀菌属等病原真菌的相对丰度与土壤pH呈负相关,与土壤速效钾含量呈正相关.本研究表明彭州大蒜根腐病发病与土壤微生物群落结构改变、土壤微生态失衡有关,结果对探明病害的发生机理具有现实意义,可为有效防治大蒜根腐病提供理论依据.(图2表5参36)To explore differences in the soil bacteria and fungi communities of healthy and root rot garlic soil in Pengzhou,healthy and root rot garlic soil samples from different sites were collected,and the microbial community structures were analyzed by Illumina Miseq sequencing technology.Proteobacteria and Chloroflexi were the dominant bacteria,and Ascomycota and Basidiomycota were the dominant fungi.Compared to healthy soils,the bacterial diversity in garlic root rot soils decreased,while the fungal diversity and abundance increased.At the genus level,the abundance of beneficial bacteria(Nitrospira,Nitrosomonadaceae_uncultured,Bacillus,and Sphingomonas)and beneficial fungi(Penicillium,Trichoderma,and Thermomyces)decreased in garlic root rot soil,while the abundance of pathogenic fungal species(Stemphylium and Fusarium)increased.Spearman analysis showed that the abundance of pathogenic fungi was negatively correlated with soil pH,but was positively correlated with available potassium in the soil.These results suggest that the incidence of garlic root rot is related to variations in the Pengzhou soil microbial community structure and micro-ecological imbalances.

关 键 词：大蒜 根腐病 土壤微生物群落 高通量测序 

分 类 号：S154.3[农业科学—土壤学] S436.33[农业科学—农业基础科学]