冀东地区甘薯种苗的茎尖脱毒技术  

Studies on Stem Tip Virus-Elimination Technique in Sweet Potato in Eastern Hebei Province

作　　者：徐燕 垢志霞 韩金玲 俎天娇 王艳敏 乔亚科 李桂兰 XU Yan;KOU Zhixia;HAN Jinling;ZU Tianjiao;WANG Yanmin;QIAO Yake;LI Guilan(College of Agronomy and Biotechnology,Hebei Normal University of Science&Technology,Qinhuangdao Hebei,066600;Institute of Sweet Potato Industry Technology in Hebei Province,China)

机构地区：[1]河北科技师范学院,农学与生物科技学院,河北秦皇岛066600 [2]河北省(秦皇岛)甘薯产业技术研究院

出　　处：《河北科技师范学院学报》2020年第2期19-24,共6页Journal of Hebei Normal University of Science & Technology

基　　金：秦皇岛市科学技术研究与发展计划项目(项目编号:201801B026,201903B010,201803B003)。

摘　　要：以3个甘薯品种(北京553,烟薯25,卢选1号)为试验材料,研究外植体消毒和不同激素配比对茎尖诱导的影响。以甘薯茎尖分生组织为培养对象,筛选出适合甘薯不同品种的消毒方法和适合外植体生长的激素配方。结果表明,烟薯25,卢选1号,北京553分别用ρ(NaClO)=20.0 g·L^-1消毒9,13,11 min污染率最低,成苗率最高。适合烟薯25,卢选1号和北京553的最佳培养基分别是MS+1.5 mg/L 6-BA+0.1 mg/L IBA,MS+1.0 mg/L 6-BA+0.1 mg/L IBA和MS+1.0 mg/L 6-BA+0.5 mg/L IBA。Three sweet potato varieties,including Beijing-553,Yanshu-25 and Luxuan-1,were used as materials to reveal the effects of explant sterilization and different hormone combinations on the induction of shoot tip.The meristem of the stem tip of sweet potato was used as the object of culture to select suitable disinfection method for various varieties of sweet potato and hormone combination for explant growth.The results showed that the contamination rate was the lowest and the seedling rate was the highest when Yanshu-25,Luxuan-1 and Beijing-553 were sterilized byρ(NaClO)=20.0 g·L^-1 for 9 min,13 min and 11 min,respectively.The best culture medium for Yanshu-25,Luxuan-1 and Beijing-553 were MS+1.5 mg/L 6-BA+0.1 mg/L IBA,MS+1.0 mg/L 6-BA+0.1 mg/L IBA,MS+1.0 mg/L 6-BA+0.5 mg/L IBA,respectively.

关 键 词：甘薯 茎尖培养 脱病毒苗 冀东地区 

分 类 号：S531[农业科学—作物学]