混合无机氮源下6株微藻对亚硝态氮、氨氮净化规律初探  

Nitrite and Ammonia Removal Patterns of 6 Species or Genera of Microalgae Exposed to Mixed Inorganic Nitrogen

作　　者：苟万里 李自英 武心华 文双喜 杨智 GOU Wanli;LI Ziying;WU Xinhua;WEN Shuangxi;YANG Zhi(College of Biological and Environmental Engineering, Guiyang University, Guiyang 550005, China;Qingdao Vland Biotech Incorporation, Qingdao 266000, China)

机构地区：[1]贵阳学院环境与生物工程学院,贵州贵阳550005 [2]青岛蔚蓝生物集团股份有限公司,山东青岛266000

出　　处：《水产科学》2020年第5期685-693,共9页Fisheries Science

基　　金：贵阳市科技局项目(GYU-KYZ[2018]02-16);贵州省林业厅项目(K18200022)。

摘　　要：以混合无机氮源(亚硝态氮+硝态氮+氨氮)配制培养基,在25℃条件下培养6株微藻(3株硅藻、3株绿藻),通过分析各藻株的细胞密度、氨氮质量浓度、亚硝态氮质量浓度的变化情况,研究微藻在混合无机氮源下对氨氮和亚硝态氮的净化规律。试验结果显示,所有藻在整个试验期均生长良好,经历了一个相对完整的生长周期。在生长周期的初期(培养至第2~3 d),所有藻均能净化95%以上的氨氮,但各株藻对氨氮的相对净化速率并不相同,以塔胞藻KDN21的最高[0.999 mg/(L·d)],三角褐指藻KDN13的最低[0.663 mg/(L·d)];大部分微藻对亚硝态氮的净化能力很弱,相对净化率均小于35%,相对净化速率均未超过0.035 mg/(L·d),双眉藻KDN17能较快地净化亚硝态氮,其相对净化率和相对净化速率分别达到65%和0.322 mg/(L·d)。在生长周期的中后期,微藻对亚硝态氮的净化能力仍然很弱,大部分藻株的相对净化速率均低于0.010 mg/(L·d)。研究结果表明,当水体中的氮源仅有氨氮、亚硝态氮和硝态氮时,且总氮能满足藻细胞充分生长的条件下,绝大部分微藻都优先净化氨氮,而对亚硝态氮的净化能力始终很弱;双眉藻KDN17是6株藻中唯一能同时快速净化氨氮和亚硝态氮的藻株,有可能成为人工控制水体氨氮和亚硝态氮含量的候选藻株。The nitrite and ammonia removal patterns of 6 microalgal species or genera,including 3 diatoms,and 3 members in chlorophyto were primarily studied by analyzing the variation trend of cell density,nitrite concentration,and the ammonia concentration in the modified F/2 medium whose nitrogen source was the mixture of ammonia,nitrate and nitrite.The results showed that all microalgae grew very well and completed a whole growth cycle.In the initial stage of the growth cycle(2—3 d),each strain removed above 95%of the ammonia,with the maximal relative removal rate of 0.999 mg/(L·d)in Pyramimonas sp.KDN21 and the minimal relative removal rate of 0.639 mg/(L·d)in Amphora sp.KDN17.Most strains slowly removed a little nitrite(lower than 35%)during this stage,and their relative removal rates were not exceed 0.035 mg/(L·d);Amphora sp.KDN17 was the only microalga which sharply removed up to 65%of the nitrite,with relative removal rate of 0.322 mg/(L·d).In the middle to late stages of the growth cycle(3—6 d),their relative removal rates were lower than 0.020 mg/(L·d),although removed almost all NH+4-N,all strains still removed a little nitrite.These findings indicated that the majority of microalga grew on the mixture inorganic nitrogen sources composed of ammonia,nitrate and nitrite removed massive ammonia,but a little nitrite.Amphora sp.KDN17 was the only one which removed massive ammonia and nitrite simultaneously,so as a probable candidate strain for controlling the water ammonia and nitrite concentration.

关 键 词：微藻 亚硝态氮 氨氮 混合无机氮源 净化规律 

分 类 号：S963.213[农业科学—水产养殖]