早期肾缺血再灌注损伤MRI纹理分析与病理组织学对照研究  

A comparative study of MRI texture analysis and histopathology for early renal ischemia-reperfusion injury

作　　者：潘靓[1] 邢兆宇[2] 邢伟[1] 陈杰[1] 查婷婷[1] 徐亮 Pan Liang;Xing Zhaoyu;Xing Wei;Chen Jie;Zha Tingting;Xu Liang(Department of Radiology,First People′s Hospital of Changzhou,Changzhou 213003,China;Department of Urology,First People′s Hospital of Changzhou,Changzhou 213003,China)

机构地区：[1]常州市第一人民医院放射科,213003 [2]常州市第一人民医院泌尿外科,213003

出　　处：《中华放射学杂志》2020年第9期891-897,共7页Chinese Journal of Radiology

基　　金：国家自然科学基金(81771798);常州市科技计划(CJ20179021)。

摘　　要：目的探讨早期肾脏缺血再灌注损伤(IRI)MRI纹理特征与病理组织学的相关性。方法IRI组(n=40)实验兔夹闭左肾动脉60 min后松开建立肾IRI模型。于术前、术后1 h、12 h、24 h、48 h行MRI检查,每次MRI扫描后处死8只IRI组实验兔,假手术组(n=8)实验兔于术后48 h MRI扫描后处死行病理检查,计算病理组织学特征评分、微血管密度(MVD)及病理总分。采用Kruskal-Wallis检验比较各组肾脏病理组织学的差异。提取左肾MRI纹理特征,采用Pearson相关系数分析MRI纹理特征与病理组织学特征的相关性。结果术前、术后1 h、12 h、24 h、48 h IRI组肾小管刷状缘破坏、肾小管上皮细胞水肿、坏死、管型、间质炎性细胞浸润、MVD及病理总分差异均有统计学意义(P均<0.05)。T2WI_S(3,-3)InvDfMom与肾小管刷状缘破坏、肾小管上皮细胞水肿、肾小管上皮细胞坏死、肾小管管型均具有最高的相关性(r值分别为0.56、-0.58、0.62和0.69,P均<0.01)。BOLD_S(4,-4)Correlat与间质炎性细胞浸润相关性最高(r=0.63,P<0.01)。SWI_S(4,4)DifEntrp与微血管密度相关性最高(r=0.61,P<0.01)。结论MRI纹理分析能为评价早期肾脏IRI提供有价值的信息。Objective To explore the relationship between MRI texture features with the histopathology in early renal ischemia-reperfusion injury(IRI).Methods Forty-eight healthy New Zealand rabbits were randomly divided into the IRI group(n=40)and the sham group(n=8).As for the IRI group,rabbits underwent the left kidney surgery by clamping the left renal artery for 60 mins and then releasing the clamp to establish renal IRI model.The rabbits in the sham group underwent the same operation,but without clamping the left renal artery.MR examination were performed before and at 1 h,12 h,24 h,and 48 h after the operations.As for the IR group,eight rabbits were randomly sacrificed at each time point immediately after MR exam.The eight rabbits in the sham group were sacrificed after MR exam at 48 h after the operation.The left kidney was harvested for histopathological examination.The single item score of the histopathological features,the microvessel density(MVD),and the pathological total score were calculated.Differences in the single item score of the histopathological features,MVD,and the pathological total score among IRI group with different time points and sham group were determined using the Kruskal-Wallis test.MRI texture features of the left kidney were extracted.Multiple dimensionality reduction for MRI texture features were performed,and the features associated with the pathological total score were selected.The relationship between MRI texture features with the single item score of the histopathological features and MVD was assessed by Pearson correlation coefficients.Results There were significant differences in renal tubular brush border destruction,renal tubular epithelial edema,necrosis,cast,interstitial inflammatory cell infiltration,MVD,and the pathological total score in IRI group among the different time points(all P<0.05).T2WI_S(3,-3)InvDfMom had the highest correlation with renal tubular brush border destruction,renal tubular epithelial edema,necrosis,and cast(r=0.56,-0.58,0.62,0.69,all P<0.01).BOLD_S(4,-4)

关 键 词：肾脏 缺血再灌注损伤 磁共振成像 纹理分析 

分 类 号：R692[医药卫生—泌尿科学]