Multienzyme co-immobilization-based bioelectrode:Design of principles and bioelectrochemical applications  

作　　者：Ranran Wu Haiyan Song Yuanming Wang LeiWang Zhiguang Zhu 

机构地区：[1]Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,32West Seventh Avenue,Tianjin Airport Economic Area,Tianjin 300308,China [2]University of Chinese Academy of Sciences,19A Yuquan Road,Shijingshan District,Beijing 100049,China [3]National Human Genetic Resource Center,12 Dahuisi Road,Haidian District,Beijing 100081,China

出　　处：《Chinese Journal of Chemical Engineering》2020年第8期2037-2050,共14页中国化学工程学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(21878324,21706273);the CAS Pioneer Hundred Talent Program(Type C,reference#2016-081)。

摘　　要：Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermediate transfer among different enzymes and electron transfer from enzyme active sites to the electrode with high stability and retrievability. Different co-immobilization strategies to construct multienzyme bioelectrodes have been widely reported, however, up to now, they have barely been reviewed. In this review, we focus on recent state-of-the-art techniques for constructing co-immobilized multienzyme electrodes including random and positional co-immobilization. Particular attention is given to strategies such as multienzyme complex and surface display. Cofactor co-immobilization on the electrode is also crucial for the enhancement of catalytic reaction and electron transfer, yet, few studies have been reported. The up-to-date advances in bioelectrochemical applications of multienzyme bioelectrodes are also presented. Finally, key challenges and future perspectives are discussed.

关 键 词：Multienzyme co-immobilization Bioelectrode Bioelectrochemical system Cascade reaction 

分 类 号：TQ426.97[化学工程] O646.5[理学—物理化学]