一个大豆雄性不育雌性可育突变体的遗传稳定性及天然异交特性评价  

Assessment of Genetic Stability and Natural Cross Pollination for A Male Sterile-Female Fertile Mutant of Soybean

作　　者：张湘 高华伟[1,2,3] 杨梦园 王俊 张玉[6] 樊颖伦 苏欢[1,8] 刘立科[1] ZHANG Xiang;GAO Hua-wei;YANG Meng-yuan;WANG Jun;ZHANG Yu;FAN Ying-lun;SU Huan;LIU Li-ke(School of Life Sciences,Liaocheng University,Liaocheng 252059,China;Key Laboratory of Soybean Biology of Chinese Education Ministry,Northeast Agricutural University,Harbin 150030,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Soybean Biology(Beijing)of the Ministry of Agriculture,Bejing 100081,China;Henan Tuoren Medical Device Co.,Ltd.,Xinxiang 453400,China;College of Agriculture,Y angtze University,Jingzhou 434025,China;Shandong Xinfeng Seeds Co.,Lud.,Liaocheng 252400,China;College of Agronomy,Liaocheng University,Liaocheng 252059,China;Wenyuan Senior High school,Liaocheng 252000,China)

机构地区：[1]聊城大学生命科学学院,山东聊城252059 [2]东北农业大学大豆生物学省部共建教育部重点实验室,黑龙江哈尔滨150030 [3]中国农业科学院作物科学研究所/农作物基因资源与遗传改良国家重大科学工程/农业部北京大豆生物学重点实验室,北京100081 [4]驼人集团,河南新乡453400 [5]长江大学农学院,湖北荆州434025 [6]山东鑫丰种业股份有限公司,山东聊城252400 [7]聊城大学农学院,山东聊城252059 [8]聊城市文苑高中,山东聊城252000

出　　处：《大豆科学》2020年第4期527-534,共8页Soybean Science

基　　金：国家“十三五”重点研发计划(2016YFD0100201-09);农业部国家作物种质资源保护专项(2015NWB030-05)。

摘　　要：为了提高大豆品种培育过程中轮回选择的效率,本研究以1个大豆雄性不育-雌性可育突变体M4~M8和M12代育性分离群体为材料,分别5年在1个地点和1年在2个地点对其育性遗传方式进行分析。同时,以该突变体天然杂交F1单株衍生的5个F2群体和3个F2衍生的F3群体为材料对育性表型遗传方式和不育株结荚数进行统计分析。结果表明:在M4~M8和M12代群体中,突变性状均为单基因控制的隐性性状;而在天然杂交组合后代群体中,育性表型的遗传因组合和播期不同而异。两个F2群体的育性表现为受单基因控制,且其衍生的F3群体育性在不同播期条件下也表现为受单基因控制;而其它3个F2群体则表现为受2个基因控制,但其中1个群体衍生的F3群体在早播条件下却表现为受单基因控制。基本农田环境下,F2群体中不育株结荚数范围为0~28个,平均2.73个。在不同遗传背景下,不同播期和年际间,不育株结荚数均差异显著。本研究结果能够为该突变体用于大豆轮回选择奠定基础。In order to improve the efficiency of recurrent selection in soybean breeding, genetic stability of a male sterile-female fertile mutant was assessed through five years at one location in populations of M4 to M8 segregation populations and one year at two locations in M12segregation populations derived from heterozygous mutant plants. At the same time, natural pollination and genetic characters of the mutant allele under other genetic backgrounds were measured in five F2 populations which were derived from five naturally pollinated F1 plants and three F2 derived F3 populations. F2 populations were planted in farmland with normal soil fertility in 2017, and three F3 populations were planted on three different dates in a field with poor soil fertility in 2019.The results showed that in M4-M8 and M12 populations, male sterility of this mutant was controlled by one pair of recessive alleles at Liaocheng, Shandong province through six years(from 2011 to 2015 and 2016) and one year at Jingzhou, Hubei province in 2019. In two of five F2 populations in 2017 and those derived F3 populations in 2019, the ratios of male fertility to male sterility were both 3∶1, indicating the male sterility was controlled by a pair of recessive alleles. In other three F2populations, however, the phenotype showed a ratio of 9∶7 in 2017 indicating male fertility was controlled by two genes. In two of these three derived F3populations in 2019 the phenotype had the same genetic characters through all the planting dates as those in 2017. However, in last one of these three derived F3populations the male sterility was controlled by one gene when planted early, while by two genes when planted on the other two dates. In F2 populations, the pod number per male sterile plants ranged from 0 to 28 with a mean of 2.73 when planted in normal farmland in 2017. Pod number per male sterile plant varied significantly among different naturally pollinated crosses, planting dates, and years. The results in this research would set a solid foundation for soy

关 键 词：大豆 雄性不育 轮回选择 遗传稳定性 天然异交率 

分 类 号：S565.1[农业科学—作物学]