巨大芽孢杆菌Sneb207诱导大豆抗胞囊线虫病的防效及光合响应  

Prevention Effect and Photosynthetic Performance in Bacillus Megaterium Sneb207 Against Soybean Cyst Nematode

作　　者：周园园 郭永霞[1,3,4] 段玉玺[2] 李海燕 陈立杰[2] ZHOU Yuan-yuan;GUO Yong-xia;DUAN Yu-xi;LI Hai-yan;CHEN Li-jie(Agricultural College,Heilongjiang Bayi Agricultural University,Daqing 163316,China;College of Plant Protection,Shenyang Agricultural University/Nematology Institute of Nothern China,Shenyang 110866,China;Heilongjiang Province Cultivating Collaborative Innovation Center for the Beidahuang Modern Agricultural Industry Technology,Daqing 163316,China;Heilongjiang Provincial Key Laboratory of Crop-Pest Interaction Biology and Ecological Control,Daqing 163316,China)

机构地区：[1]黑龙江八一农垦大学农学院,黑龙江大庆163316 [2]沈阳农业大学植物保护学院/北方线虫研究所,辽宁沈阳110866 [3]北大荒现代农业产业技术省级培育协同创新中心,黑龙江大庆163316 [4]黑龙江省作物-有害生物互作生物学及生态防控重点实验室,黑龙江大庆163316

出　　处：《大豆科学》2020年第4期605-611,共7页Soybean Science

基　　金：国家自然科学基金重点项目(31330063);国家自然基金(31171569);现代农业产业技术体系岗位科学家专项(CARS-04-PS13);黑龙江八一农垦大学学成、引进人才科研启动项目(XYB201904);黑龙江省应用技术研究与开发计划项目(GA19B104)。

摘　　要：为明确巨大芽孢杆菌Sneb207对大豆抗胞囊线虫的防效及对大豆早期光合作用的影响,采用盆栽试验,通过Sneb207发酵液包衣感病大豆品种,以无菌水包衣为对照,接种大豆胞囊线虫,于显囊期调查植株生长指标及Sneb207对胞囊的防效,并且分别于接种线虫后3,7,10和14 d测定Sneb207包衣处理和对照处理大豆的光合性能及叶绿素含量。结合前期转录组分析结果,选择4个与光合作用或叶绿体相关基因进行qRT-PCR验证。结果表明:Sneb207包衣处理,大豆主根长增加且须根数量增多,同时提高了对胞囊的防效,根上和土中胞囊抑制率分别达到44.93%和57.19%。Sneb207包衣处理提高了大豆的净光合速率和叶绿素含量。所检测的4个基因在Sneb207诱导大豆抗胞囊线虫过程中2个被诱导、1个被抑制,且与对照处理差异显著,表明它们可能在Sneb207诱导大豆抗胞囊线虫中起作用。To determine the effectiveness of Bacillus megaterium Sneb207 against soybean cyst nematodes and early photosynthesis in soybean, the seed coating technology was used to coat the susceptible soybean varieties, and the sterile water coating was used as a control treatment in greenhouse experiments. We surveyed the cysts on the soybean roots and in the rhizosphere soil, the influence on soybean growth after inoculation 30-35 d, and measured the net photosynthetic rate(Pn), stomatal conductance(Gs), transpiration rate(Tr), intercellular CO2 concentration(Ci) and chlorophyll content after inoculated 3, 7, 10 and 14 d. We found that, in the Sneb207 coated and nematode inoculated treatment, the main root length and the number of fibrous roots of soybean were increased, and the soybean cyst nematode outside the soybean roots and in rhizosphere soil were suppressed, the inhibition rates of cysts reached 44.93% and 57.19%, respectively. Further, the Sneb207 coated treatment increased the Pn and chlorophyll content. In addition, in the four genes, there were two genes up-regulated and one gene down-regulated expression when the resistance to SCN of soybean was induced by Sneb207, and there were significant difference with control, indicating that they may play a role in Sneb207-induced resistance to SCN of soybean.

关 键 词：巨大芽孢杆菌Sneb207 大豆胞囊线虫 光合性能 差异表达基因 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]