徐豆24大豆根瘤菌共生匹配性筛选及应用  

Screening and Application of Suitable Symbiotic Combination Between Rhizobia and Soybean Cultivar Xudou 24

作　　者：李彦连[1] 王传雷 徐保民 张修国[4] 魏祥圣 杨升辉 LI Yan-lian;WANG Chuan-lei;XU Bao-min;ZHANG Xiu-guo;WEI Xiang-sheng;YANG Sheng-hui(Department of Life Science and Engineering,Jining University,Qufu 273155,China;Jining Rencheng Bureau of Science and Technology,Jining 272000,China;Jining Bureau of Agriculture and Rural Affairs,Jining 272000,China;College of Plant Protection,Shandong Agricultural University,Tai′an 271000,China;Shandong Aifudi Biotech.Co.,LTD.,Jining 272000,China)

机构地区：[1]济宁学院生命科学与工程系,山东曲阜273155 [2]济宁市任城区科学技术局,山东济宁272000 [3]济宁市农业农村局,山东济宁272000 [4]山东农业大学植物保护学院,山东泰安271000 [5]山东爱福地生物股份有限公司,山东济宁272000

出　　处：《大豆科学》2020年第4期612-620,共9页Soybean Science

基　　金：山东省重点扶持区域引进急需紧缺人才项目(SF1804002003);济宁市重点研发计划(2019ZDGH017)。

摘　　要：为研究大豆与根瘤菌之间的共生匹配性、筛选具有应用潜力的大豆根瘤菌,在黄淮海地区主栽大豆品种徐豆24的种植田中捕捉、分离得到32株根瘤菌,筛选与徐豆24共生匹配的高效根瘤菌株,再通过田间回接试验,研究施肥及接种高效根瘤菌对徐豆24产量及品质的影响。经rpoB基因序列比较鉴定,这些根瘤菌分别分属于Sinorhibizobiumfredii、Bradyrhizobium elkanii和Bradyrhizobium japonicum种群。其中,S.fredii的占有率达到了75%。菌株共生匹配筛选结果确定S.fredii3为与徐豆24相匹配的高效菌株。田间回接试验结果进一步证明S.fredii3是试验地区的优势种,M2处理(施底肥、接种S.fredii3、不追肥)的根瘤数、根瘤鲜重、产量和蛋白含量均最高。根瘤数和根瘤鲜重与产量和蛋白含量均呈正相关,其中,根瘤鲜重与产量达到了显著性正相关。土壤有效硼含量与S.fredii的群落分布的相关性最大,有效铁和有效钼含量是影响B.elkanii群落结构分布的最重要的理化因子。研究表明,S.fredii3菌株可作为黄淮海地区徐豆24的高效接种剂。In order to study the symbiotic matching between the local soybean cultivar and rhizobial species, the main soybean variety Xudou 24 cultured in Huanghuaihai area was used in this experiment to trap rhizobia in the fields. We screened high efficient rhizobium strains matched with Xudou 24 symbiosis and studied the effects of fertilization and inoculation with efficient rhizobia on yield and quality of Xudou 24 by inoculated experiment in field. A total of 32 rhizobial strains were isolated from the trapping plants. These strains were identified as three populations belonging to the species Sinorhibizobium fredii, Bradyrhizobium elkanii and Bradyrhizobium japonicum by rpoB gene sequence comparison, in which S.fredii was the dominant with 75% of nodule occupation. Through the symbiotic matching screening,strain S.fredii 3 was verified as a high-efficiency symbiont of Xudou 24. The field inoculation experiments further proved that the inoculation of S.fredii 3 could improve the growth and yield of Xudou 24 and the number of nodules, fresh weight of nodules, yield and protein content of the plant in M2 treatment(base fertilizer, inoculation with S.fredii 3, non-topdressing) were the highest.Further analysis found that the number of nodules and fresh weight of nodules were positively correlated with the yield and protein content. Among them, the fresh weight of nodules and yield reached a significant positive correlation. The content of soil available boron(AB) was the most related to the community distribution of S.fredii, and the content of available iron(AFe) and available molybdenum(AMo) were the most important physicochemical factors affecting the community structure distribution of B.elkanii. It is showed that S.fredii 3 could be a suitable candidate for inoculant of Xudou 24 in the Huanghuaihai area.

关 键 词：徐豆24 黄淮海 根瘤菌 共生匹配 产量 品质 

分 类 号：S565.1[农业科学—作物学]