紫花地丁多糖对A549细胞增殖抑制机理的研究  

Inhibitory mechanism of Viola philippica polysaccharide on A549 cells

作　　者：袁会成 赵良存 万学娜 董吉虎 YUAN Hui-cheng;ZHAO Liang-cun;WAN Xue-na;DONG Ji-hu(Gansu Wuwei Tumor Hospital,Wuwei,Gansu 733000,China)

机构地区：[1]甘肃省武威肿瘤医院,甘肃武威733000

出　　处：《热带医学杂志》2020年第8期1005-1008,共4页Journal of Tropical Medicine

基　　金：武威市科技计划项目(WW160202)。

摘　　要：目的研究紫花地丁多糖(VPPS)对A549细胞直接抑制作用。方法以不同浓度VPPS(40、80、160、320、480μg/mL)为试验组,设空白对照。采用CCK-8实验和克隆形成实验观察VPPS对A549细胞的抑制作用;采用流式细胞仪检测VPPS对A549细胞周期分布及细胞凋亡的影响。结果 VPPS(40、80、160、320、480μg/mL)试验组对人肺腺癌A549细胞克隆形成存活分数分别为(51.4±3.3)%、(28.3±3.3)%、(25.0±6.0)%、(20.6±1.7)%、(23.7±2.3)%;增殖抑制率分别为(22.5±5.7)%、(30.1±5.5)%、(44.9±7.8)%、(52.8±8.5)%、(65.1±9.6)%。VPPS试验组(40、80、160、320、480μg/mL)在G2/M期占细胞周期的比例分别为(4.7±0.5)%、(7.4±0.4)%、(8.2±0.7)%、(11.0±1.1)%、(14.4±0.5)%,均高于对照组(2.9±0.3)%,差异有统计学意义(P均<0.05)。试验组(80、160、320、480μg/mL VPPS)对A549细胞凋亡率分别为(38.7±5.5)%、(49.3±7.8)%、(54.8±7.8)%、(61.1±8.2)%,均高于对照组(15.1±0.4)%,差异有统计学意义(P均<0.05)。结论 VPPS通过诱导肿瘤细胞A549凋亡直接发挥抑制肿瘤作用。Objective The direct inhibition of A549 cells by Viola philippica polysaccharide(VPPS) was studied.Methods Different concentrations of VPPS(40,80,160,320,480 μg/mL)were used in the experimental group,and a blank control was set.The inhibitory effect of VPPS on A549 cells was observed by CCK-8 assay and colony formation assay.The effect of VPPS on cell cycle distribution and apoptosis of A549 cells was detected by flow cytometry.Results The survival rates of human lung adenocarcinoma A549 cells treated with 40,80,160,320 and 480 μg/mL of VPPS were(51.4±3.3)%、(28.3±3.3)%、(25.0±6.0)%、(20.6±1.7)%、(23.7±2.3)%,respectively,and the proliferation inhibition rates were(22.5±5.7)%、(30.1 ±5.5)%、(44.9±7.8)%、(52.8±8.5)%、(65.1±9.6)%.The proportion of the cell cycle in the G2/M phase of the control group was(2.9±0.3)%,and those of the test group at different VPPS concentrations、were(4.7±0.5)%,(7.4±0.4)%,(8.2±0.7)%,(11.0±1.1)% and(14.4±0.5)%,respectively.Compared with the control group,the differences were statistically significant(P<0.05).The apoptosis rate of A549 cells of the control group was(15.1±0.4)%,and those of the test groups treated with different VPPS concentrations were(38.7±5.5)%,(49.3±7.8)%,(54.8±7.8)% and(61.1±8.2)%.Compared with the control group,the differences were statistically significant(P<0.05).Conclusion VPPS could inhibit proliferation and induce apoptosis in A549 cells.

关 键 词：紫花地丁多糖 A549 CCK-8 细胞凋亡 

分 类 号：R965.2[医药卫生—药理学]