Tim3/Galectin-9在肺结核患者中的表达研究  

Study on Tim3/Galectin-9 in pulmonary tuberculosis

作　　者：穆合塔尔·木合买提 李奇凤[2] 赵晶[2] 余亮[2] MUHETAER Muhemaiti;LI Qi-feng;ZHAO Jing;YU Liang(Department of Pediatrics,People′s Hospital of Xinjiang Uygur Autonomous Region,Urumqi,Xinjiang 830001;Xinjiang Institute of Pediatrics,People′s Hospital of Xinjiang Uygur Autonomous Region,Urumqi,Xinjiang 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院儿科,新疆乌鲁木齐830001 [2]新疆维吾尔自治区人民医院,新疆儿科研究所,新疆乌鲁木齐830001

出　　处：《热带医学杂志》2020年第8期1033-1035,1055,共4页Journal of Tropical Medicine

基　　金：新疆维吾尔自治区自然科学基金(2018D01C093);新疆维吾尔自治区人民医院院内项目(20190115)。

摘　　要：目的研究负性协同刺激分子T细胞免疫球蛋白黏蛋白分子3(Tim3)/半乳凝素9(Galectin-9)在肺结核患者中的表达情况,探讨其在肺结核发生中的免疫作用。方法收集2018年1-10月确诊的肺结核患者94例,结核菌潜伏感染者33例和未感染者26例,取外周血2 mL,应用ELISA法检测血清中sTim-3/Galectin-9水平。结果 94例肺结核患者中,初治51例,复治43例。与未感染组[0.422(0.143,1.090)]和潜伏感染组[0.181(0.045,0.689)]比较,肺结核组[3.538(1.237,32.980)]血清中sTim-3水平明显升高,差异有统计学意义(P<0.05),复治组[19.110(2.603,83.650)]血清中sTim-3水平高于初治组[2.267(0.641,5.282)],差异有统计学意义(P<0.05);与未感染组[541.1(351.2,601.2)]和潜伏感染组[468.2(384.3,637.0)]分别比较,肺结核组[691.1(446.8,1 705.0)]Galectin-9水平明显升高,差异有统计学意义(P<0.05),复治组[1 233.0(519.5,3 700.0)]血清中Galectin-9水平明显高于潜伏感染组和初治组[531.8(395.6,835.4)],差异有统计学意义(P<0.05)。肺结核患者血清中sTim-3和Galectin-9为正相关(r=0.609、P=0.000)。结论肺结核患者血清中sTim-3/Galectin-9水平在不同疾病状态时存在差异,提示sTim-3/Galectin-9在疾病的进展中起非常重要的作用,为进一步深入研究肺结核发病机制奠定了基础。Objective To study the expression of negative costimulatory molecule T cell immunoglobulin mucin molecule 3(Tim3)/Galectin-9 in patients with tuberculosis and explore its immunological role in the occurrence of tuberculosis.Methods 94 cases of tuberculosis,33 cases of latent tuberculosis infection and 26 cases of uninfected tuberculosis were collected from January to October 2018.The serum Tim3(sTim-3)/Galectin-9 level "was detected by ELISA.Results Among94 patients with pulmonary tuberculosis,51 were initially treated and 43 were retreated.Compared with the uninfected group [0.422(0.143,1.090)] and in the latent tuberculosis infection group [0.181(0.045,0.689)],the serum level of serum Tim3 in the tuberculosis group [3.538(1.237,32.980)] increased significantly(P<0.05).The serum Tim3 level in the retreatment group [19.110(2.603,83.650)] was higher than that in the initial treatment group [2.267(0.641,5.282)](P<0.05).Compared with the uninfected group [541.1(351.2,601.2)] and in the latent infection group [468.2(384.3,637.0)],the serum level of Galectin-9 was significantly higher in tuberculosis group [691.1(446.8,1 705.0)](P<0.05).The serum level of Galectin-9 in retreatment group was higher [1 233.0(519.5,3 700.0)] than those in latent infection group and initial treatment group [531.8(395.6,835.4)](P<0.05).There was a positive correlation between serum Tim3 and Galectin 9 in patients with pulmonary tuberculosis(r=0.609,P=0.000).Conclusion The levels of serum Tim3/Galectin-9 of patients with pulmonary tuberculosis were different at different disease stages,suggesting that serum Tim3/Galectin-9 might play an important role in the progress of the disease.

关 键 词：肺结核 可溶性T细胞免疫球蛋白黏蛋白分子3 GALECTIN-9 

分 类 号：R521[医药卫生—内科学]