杜仲无性系花芽的抗寒性测定与综合评价  

Determination and comprehensive evaluation on cold resistance of flower bud in Eucommia ulmoides clone

作　　者：何凤 朱利利 刘攀峰[1,3,4] 杜庆鑫 庆军[1,3,4] 王璐 杜红岩 HE Feng;ZHU Lili;LIU Panfeng;DU Qingxin;QING Jun;WANG Lu;DU Hongyan(Non-timber Forest Research&Development Center,Chinese Academy of Forestry,Zhengzhou 450003,Henan,China;Nanjing Forestry University,Nanjing 210037,Jiangsu,China;Paulownia R&D Center of National Forestry and Grassland Administration,Zhengzhou 450003,Henan,China;Key Laboratory of Non-timber Forest Germplasm Enhancement&Utilization of National Forestry and Grassland Administration,Zhengzhou 450003,Henan,China)

机构地区：[1]中国林业科学研究院经济林研究开发中心,河南郑州450003 [2]南京林业大学,江苏南京210037 [3]国家林业和草原局泡桐研究开发中心,河南郑州450003 [4]经济林种质创新与利用国家林业和草原局重点实验室,河南郑州450003

出　　处：《中南林业科技大学学报》2020年第9期11-23,共13页Journal of Central South University of Forestry & Technology

基　　金：“十三五”国家重点研发计划项目“橡胶用和药用杜仲定向培育与高效利用关键技术研究”(2017YFD0600702);国家自然科学基金面上项目(31370682)。

摘　　要：【目的】揭示杜仲对低温胁迫的生理响应机制并评价不同杜仲无性系的抗寒能力。【方法】以20个杜仲无性系1年生休眠枝为试验材料,进行0、-10、-15、-25、-30和-40℃的人工低温处理,研究低温胁迫对不同无性系花芽的生理响应,采用隶属函数综合评价20个杜仲无性系的抗寒性。【结果】游离脯氨酸随处理温度的降低呈“升-降-升”或“升-降-升-降”的变化趋势,可溶性蛋白含量随处理温度的降低呈“升-降-升”或“降-升-降-升”的变化趋势,SOD酶活性随处理温度的降低呈“升-降-升”或“降-升-降-升”的变化趋势,POD酶活性随处理温度的降低呈“升-降-升-降”或“降-升-降”的变化趋势,CAT酶活性随处理温度的降低呈“升-降-升-降”或“降-升-降-升”的变化趋势。用隶属函数法对20个杜仲无性系的抗寒性综合评价,抗寒性为‘10493C’>‘10586C’>‘10480C’>‘10115C’>‘10046C’>‘10475C’>‘10044C’>‘10408C’>‘10472C’>‘10508C’>‘10267C’>‘10428C’>‘10012C’>‘10043C’>‘10527C’>‘10407C’>‘10476C’>‘10013C’>‘10526C’>‘10409C’。聚类分析将20种杜仲无性系耐受低温胁迫能力划分为3类,其中‘10493C’和‘10115C’耐受性较强,‘10586C’‘10475C’‘10044C’‘10267C’‘10043C’耐受性中等,其他13个杜仲无性系耐受性较弱。【结论】‘10493C’和‘10115C’可供将来在寒冷地区引种中参考使用。【Objective】In order to reveal the physiological response mechanism of Eucommia ulmoides to low temperature stress and evaluate the cold resistance of different Eucommia ulmoides clones.【Method】20 one-year dormant branches of Eucommia ulmoides were adopted as experimental materials and were subjected under artificial low temperature at 0°C,-10°C,-15°C,-25°C,-30°C and-40°C to study the physiological response of low temperature stress to flower bud of different clones.Comprehensive evaluation on cold tolerance of the 20 Eucommia ulmoides clones was conducted using membership function.【Result】The content of proline shows a trend of“increase-decrease-increase”or“increase-decrease-increase-decrease”;the content of soluble protein indicates a trend of“increase-decrease-increase”or“decrease-increase-decrease-increase”;SOD activity points a trend of“increase-decrease-increase”or“decrease-increase-decrease-increase”;POD activity displays a trend of“increase-decrease-increase-decrease”or“decrease-increasedecrease”;CAT activity manifests a trend of“increase-decrease-increase-decrease”or“decrease-increase-decrease-increase”.Cold tolerance of twenty Eucommia ulmoides clones is comprehensively evaluated using membership function,and its order is‘10493C’>‘10586C’>‘10480C’>‘10115C’>‘10046C’>‘10475C’>‘10044C’>‘10408C’>‘10472C’>‘10508C’>‘10267C’>‘10428C’>‘10012C’>‘10043C’>‘10527C’>‘10407C’>‘10476C’>‘10013C’>‘10526C’>‘10409C’.The tolerance of 20 Eucommia ulmoides clones to low temperature stress is divided into three categories by cluster analysis,among which‘10493C’and‘10115C’is stronger than that of‘10586C’,‘10475C’,‘10044C’,‘10267C’and‘10043C’,while the rest 13 Eucommia ulmoides’clones is weaker.【Conclusion】‘10493C’and‘10115C’can be used for reference in the cold areas introduction in the future.

关 键 词：杜仲 低温胁迫 生理响应 抗寒性 无性系筛选 

分 类 号：S718.43[农业科学—林学]