甲维盐和高效氯氰菊酯混配染毒对雄性小鼠睾丸生化指标及精子质量的影响  

Effects of mixture of emamectin benzoate and beta-cypermethrin on biochemical indices of testis and sperm quality in male mice

作　　者：李媛媛[1] 张媛媛 李俊霞 孔畅 迟慧敏 邢杰[2] 王飞[2] 邵丽军[2] 翟庆峰[2] LI Yuan-yuan;ZHANG Yuan-yuan;LI Jun-xia;KONG Chang;CHI Hui-min;XING Jie;WANG Fei;SHAO Li-jun;ZHAI Qing-feng(Department of Neonatology,Maternal and Child Health Hospital of Weifang Medical University,Weifang,Shandong 261011,China;School of Public Health,Weifang Medical University,Weifang,Shandong 261053,China;Weifang Municipal Center for Disease Control and Prevention,Weifang,Shandong 261061,China)

机构地区：[1]潍坊医学院妇幼保健院新生儿科,山东潍坊261011 [2]潍坊医学院公共卫生学院,山东潍坊261053 [3]潍坊市疾病预防控制中心,山东潍坊261061

出　　处：《环境与职业医学》2020年第8期804-807,共4页Journal of Environmental and Occupational Medicine

基　　金：山东省医药卫生科技发展计划项目(2016WS0670);山东省高等学校科技计划项目(J18KB120)。

摘　　要：[背景]使用混配农药进行杀虫在农业生产中已成为普遍现象,由此造成的食物农药残留问题较为突出,严重威胁人体健康。甲维盐(一种大环内酯类天然杀虫剂)和高效氯氰菊酯(一类典型的拟除虫菊酯类杀虫剂)混配杀虫是温室种植者较为常用的混配方式。[目的]探讨甲维盐和高效氯氰菊酯混配染毒对小鼠睾丸生化指标及精子质量的影响。[方法]选择40只健康10周龄清洁级雄性昆明小鼠作为实验动物,随机分为4组,每组10只:正常对照组(花生油)、甲维盐组(1/20 LD50=5.465 mg·kg^-1)、高效氯氰菊酯组(1/20 LD50=4.65 mg·kg^-1)、混配组(5.465 mg·kg^-1甲维盐+4.65 mg·kg^-1高效氯氰菊酯)。采用灌胃方法进行染毒,每日染毒1次,灌胃量为每10 g体重0.1 mL,每周染毒6 d,共计5周,累积染毒30 d。染毒结束后第2天处死动物,检测小鼠睾丸组织中丙二醛(MDA)含量以及总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-PX)、乳酸脱氢酶(LDH)、酸性磷酸酶(ACP)活性,并检测精子计数和精子畸形率。[结果]各实验组未观察到小鼠精神、活动、饮食量及体态的明显差异。各组小鼠睾丸组织中GSH-PX、T-SOD、LDH酶活性差异具有统计学意义(P<0.05),并且混配组GSH-PX活性[(117.20±51.76)U·mg^-1,以蛋白计,余同]高于甲维盐组[(71.37±11.16)U·mg^-1](q=4.08,P<0.05)、高效氯氰菊酯组[(76.84±36.29)U·mg^-1](q=3.59,P<0.05)。各组小鼠精子计数和精子畸形率差异具有统计学意义(P<0.05),并且混配组精子畸形率[(28.50±1.85)%]高于甲维盐组[(20.20±4.21)%](q=6.91,P<0.01)、高效氯氰菊酯组[(23.75±2.99)%](q=3.96,P<0.05)。析因分析发现,甲维盐和高效氯氰菊酯染毒对小鼠睾丸组织中GSH-PX酶活性的交互作用存在统计学意义(P<0.05),高效氯氰菊酯染毒对睾丸组织中T-SOD活性、LDH活性、精子畸形率和精子计数的主效应有统计学意义(P<0.05)。[结论]农药甲维盐及高效氯氰菊�[Background]Mixed pesticides are commonly used to kill pests in agricultural production,and the prominent problem of pesticide residues in food seriously threatens human health.The mixture of emamectin benzoate,a natural macrolide insecticide,and beta-cypermethrin,a typical pyrethroid insecticide,is a commonly used mixed insecticide for greenhouse farmers.[Objective]This experiment investigates the effects of mixture of beta-cypermethrin and emamectin benzoate on biochemical indices of testis and sperm quality in male mice.[Methods]Forty clean 10-week-old male Kunming mice were randomly divided into four groups,10 in each group:normal control group(peanut oil),emamectin benzoate group(1/20 LD50=5.465 mg·kg^-1),beta-cypermethrin group(1/20 LD50=4.65 mg·kg^-1),and mixed group(5.465 mg·kg^-1 emamectin benzoate+4.65 mg·kg^-1 beta-cypermethrin).The animals were administered by gavage once a day at a dose of 0.1 mL per 10 g of body weight,6 d per week,for 5 weeks,totaling 30 d of exposure.The content of malondialdehyde(MDA)and the activities of total superoxide dismutase(T-SOD),glutathione peroxidase(GSH-PX),lactate dehydrogenase(LDH),and acid phosphatase(ACP)in testis tissues,as well as sperm count and sperm deformity rate were determined on the second day after the designed intragastric administration.[Results]There were no significant differences in spirit,activity,diet,and physical appearance among the experimental groups.There were significant differences in GSH-PX,T-SOD,and LDH activities among the groups(P<0.05),and the mixed group showed a higher GSH-PX activity[(117.20±51.76)U·mg-1,in terms of protein,thereafter]than the emamectin benzoate group[(71.37±11.16)U·mg^-1](q=4.08,P<0.05)and the beta-cypermethrin group[(76.84±36.29)U·mg^-1](q=3.59,P<0.05).There were also significant differences in sperm count and sperm deformity rate among the groups(P<0.05),and the mixed group showed a higher sperm deformity rate[(28.50±1.85)%]than the emamectin benzoate group[(20.20±4.21)%](q=6.91,P<0.01)and the beta-cy

关 键 词：高效氯氰菊酯 甲维盐 精子质量 氧化损伤 生殖健康 

分 类 号：R114[医药卫生—卫生毒理学]