甲状腺干扰物 p, p'-DDE抑制Nthy-ori-3-1细胞LHX4和DIS3L蛋白表达  

Thyroid disruptor p,p'-DDE inhibited the expression of LHX4 and DIS3L protein in Nthy-ori-3-1 cells

作　　者：邹伟 李佳欣 徐芳 潘红梅 周建于 柏桦 王琦 Zou Wei;Li Jiaxin;Xu Fang;Pan Hongmei;Zhou Jianyu;Bai Hua;Wang Qi(School of Public Health,Kunming Medical University,Kunming 650500,China)

机构地区：[1]昆明医科大学公共卫生学院,650500

出　　处：《中华劳动卫生职业病杂志》2020年第8期561-565,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：云南省应用基础研究计划项目(2013FZ062)。

摘　　要：目的:观察甲状腺干扰物4,4-二氯二苯二氯乙烯(p,p’-DDE)处理甲状腺Nthy-ori-3-1细胞后,其LHX4和DIS3L mRNA水平及蛋白表达量的变化。方法:取对数生长期Nthy-ori-3-1细胞,配制0、0.5、1.0、2.0和5.0μg/ml p,p’-DDE溶液,按浓度梯度给药,处理Nthy-ori-3-1细胞。显微镜观察细胞生长状态、细胞形态。基因芯片检测基因表达谱变化,通过实时荧光定量PCR检测LHX4和DIS3L mRNA水平,通过蛋白免疫印迹法(Western blot)检测LHX4和DIS3L蛋白表达量。结果:p,p’-DDE浓度为0、0.5、1.0和2.0μg/ml时,Nthy-ori-3-1细胞均生长正常。2.0μg/ml组细胞差异基因共33个,其中,13个基因表达下调,20个基因表达上调。与对照组比较,1.0、2.0μg/ml组细胞LHX4、DIS3L蛋白表达水平明显降低,差异均有统计学意义(P<0.05),1.0μg/ml组细胞LHX4和DIS3L蛋白mRNA相对表达量降低,差异均有统计学意义(P<0.05)。结论:p,p’-DDE处理Nthy-ori-3-1细胞会影响LHX4和DIS3L蛋白表达水平。Objective To observe the changes of LHX4 and DIS3L mRNA and protein expression in Nthy-ori-3-1 cells after the treatment of thyroid disruptor p,p'-DDE.Methods Nthy-ori-3-1 cells in logarithmic growth phase were treated with 0,0.5,1.0,2.0 and 5.0μg/ml p,p'-DDE solution.The growth state and morphology of the cells were observed by microscope.The mRNA levels of LHX4 and DIS3L were detected by real-time fluorescent quantitative PCR,and the protein expression levels of LHX4 and DIS3L were detected by Western blot.Results when the concentrations of p,p'-DDE were 0,0.5,1.0 and 2.0μg/ml,Nthy-ori-3-1 cells grew normally.There were 33 differential genes in 2.0μg/ml group,among which 13 genes were down regulated and 20 genes were up-regulated.Compared with the control group,the protein expression levels of LHX4 and DIS3L in 1.0 and 2.0μg/ml groups were significantly decreased(P<0.05),and the relative expression levels of LHX4 and DIS3L protein mRNA in 1.0μg/ml group were significantly decreased(P<0.05).Conclusion p,p'-DDE can affect the protein expression of LHX4 and dis3l in nthy-ori-3-1 cells.

关 键 词：4 4-二氯二苯二氯乙烯 甲状腺细胞Nthy-ori-3-1 LHX4蛋白 DIS3L蛋白 

分 类 号：R581[医药卫生—内分泌]