基于液相色谱-质谱联用的清肺排毒汤中痕量马兜铃酸I的监测及定量分析  

Monitoring and quantitative analysis of trace aristolochic acid I in a Qing-Fei-Pai-Du decoction using liquid chromatographymass spectrometry

作　　者：陈奕君 王伟[2] 肖红斌[1,2] CHEN Yi-jun;WANG Wei;XIAO Hong-bin(Research Center of Chinese Medicine Analysis and Transformation,Beijing University of Chinese Medicine,Beijing 100029,China;Beijing Key Laboratory of Syndrome Prescription Basic Research,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,中药分析与成果转化研究中心,北京100029 [2]北京中医药大学,北京市方剂与证候研究重点实验室,北京100029

出　　处：《药学学报》2020年第8期1903-1907,共5页Acta Pharmaceutica Sinica

基　　金：国家重大专项新药创制(2018ZX09101-002,2019ZX09201004-001);国家自然科学基金项目(81774155,81803703)。

摘　　要：清肺排毒汤中细辛用量超《中国药典》规定量一倍,其限量成分马兜铃酸I是否也超标引发广泛关注。本研究首先利用超高效液相色谱-四级杆飞行时间串联质谱联用技术,证实了清肺排毒汤中确有马兜铃酸I;基于此,进一步构建了高灵敏的超高效液相色谱-三重串联四级杆质谱方法,对清肺排毒汤、细辛水煎液及细辛70%甲醇提取液中的马兜铃酸I进行了定量对比分析,发现细辛用量均为6 g时,其所含马兜铃酸I的量分别约为1.5、3.2和9.0μg,均远低于《中国药典》中规定的每日最大限量(30μg)。因此,本研究推测通过复方煎煮及优选细辛药材的方法,可以有效的降低细辛中马兜铃酸I的浸出,从而避免超限使用。本研究不仅可为中药复方汤剂中痕量马兜铃酸I的检测及定量分析提供方法,也可为清肺排毒汤中细辛的合理使用提供科学依据。The dosage of asarum in Qing-Fei-Pai-Du decoction(QFPD)is twice the dosage prescribed by the Chinese Pharmacopoeia.Due to the potential toxicity of aristolochic acid I(AAI),a limited component in asarum,the possibility that its dosage also exceeds the dosage prescribed by the Chinese Pharmacopoeia had aroused wide concern.In this study,the UHPLC-Q TOF method was used to determine the presence of AAI in QFPD.A UHPLCQQQ method was then established to determine the content of AAI in QFPD,a reflux extract of asarum and an ultrasonic extract of 70%methanol of asarum.The results showed that the amount of AAI in the three samples was approximate 1.5,3.2 and 9.0μg respectively with equivalent dosages of asarum(6 g).All were obviously lower than the maximum daily limit stipulated in the Chinese Pharmacopoeia(30μg).Therefore,we concluded that the content of AAI could be effectively reduced by using a Chinese herbal compound decoction and optimization of asarum.This assay is not only convenient,rapid,sensitive and reproducible for the trace detection of AAI in Chinese herbal compound decoction,but also useful for the rational application of asarum in QFPD.

关 键 词：清肺排毒汤 细辛 马兜铃酸Ⅰ 痕量检测 

分 类 号：R917[医药卫生—药物分析学]