戊吡虫胍对棉铃虫中枢神经细胞电压门控钙通道和钾通道的影响  

Effects of Guadipyr on voltage-gated calcium and potassium channels in central neurons of Helicoverpa armigera

作　　者：关丹阳 姜笑维 李清亚 刘晓 马永强[2] 陈强[1] Li-Byarlay Hongmei 贺秉军[1] GUAN Dan-Yang;JIANG Xiao-Wei;LI Qing-Ya;LIU Xiao;MA Yong-Qiang;CHEN Qiang;LI-Byarlay Hongmei;HE Bing-Jun(The Key Laboratory of Bioactive Materials of Ministry of Education,College of Life Sciences,Nankai University,Tianjin 300071,China;Department of Applied Chemistry,China Agricultural University,Beijing 100093,China;Agricultural Research and Develop Program,Department of Agriculture and Life Science,Central State University,Wilberforce OH 45384,U.S.A)

机构地区：[1]南开大学生命科学学院,生物活性材料教育部重点实验室,天津300071 [2]中国农业大学理学院应用化学系,北京100193 [3]中央州立大学农业与生命科学系农业研究与开发计划,Wilberforce OH 45384

出　　处：《应用昆虫学报》2020年第4期841-849,共9页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金(31871992,31371974)。

摘　　要：【目的】戊吡虫胍是将新烟碱类和缩氨脲类杀虫剂杀虫活性部分重新组合的新型杀虫剂。但对于该类杀虫剂究竟如何影响离子通道,通道门控特性和功能是如何变化目前尚未见报道。本实验旨在明确该杀虫剂是否影响电压门控钙通道和钾通道的门控过程,探究其是否为该杀虫剂的潜在作用靶标。【方法】应用全细胞膜片钳技术检测戊吡虫胍对棉铃虫Helicoverpa armigera Hübner中枢神经细胞电压门控Ca^2+通道和K^+通道的影响。【结果】戊吡虫胍作用后Ⅰ-Ⅴ曲线和激活曲线均向超极化方向移动10-15 mV,具有显著性统计学差异(P<0.05)。稳态失活曲线向超极化方向移动约5 mV,不具有统计学差异(P>0.05)。电压门控Ca^2+通道峰值电流(Ipeak)有不同程度的降低。随着浓度增大Ipeak降低有减小的趋势。此外,1μmol·L^-1戊吡虫胍作用后钙离子的窗口电流(Iw)面积增加幅度较10μmol·L^-1和100μmol·L^-1大,为93.20%。提示在一定的测试电压下,该药物作用后处于激活状态的Ca^2+通道数目增多。另外,其作用后电压门控钾通道Ipeak降低。随着浓度增大Ipeak降低有减小的趋势。同时Ⅰ-Ⅴ曲线下移,激活曲线向去极化方向移动约8 mV,不具有统计学差异(P>0.05)。这表明戊吡虫胍作用后K^+通道在较高电位下才能激活。【结论】戊吡虫胍能够有效抑制Ca^2+通道和K^+通道Ipeak,并使通道的激活曲线和失活曲线发生移动,影响Ca^2+通道和K^+通道的门控特性。表明棉铃虫中枢神经细胞上的电压门控Ca^2+通道和K^+通道是戊吡虫胍的潜在作用靶标之一。[Objectives]Guadipyr is a novel insecticide that has both neonicotinoid and semicarbazone insecticidal activity.How it affects ion channels and channel gating characteristics and functions is currently unknown.We examined the effects of Guadipyr on voltage-gated calcium and potassium channels,and explored whether these channels are potential targets for this pesticide.[Methods]The effects of three concentrations(1,10,and 100μmol·L^-1)of Guadipyr on Ca^2+and K^+ channels were studied using the patch clamp technique.[Results]All three concentrations of Guadipyr significantly(P<0.05)shifted Ca^2+channelⅠ-Ⅴand activation curves about 10-15 mV in the negative direction.The inactivation curves of Ca^2+channels were also shifted 5 mV towards the hyperpolarizing direction,which is a not significant shift(P>0.05).The Ca^2+peak current was significantly decreased by Guadipyr treatment relative to the control.The Ipeak decreased more slowly as the concentration of Guadipyr increased.The area of window current increased the most(93.20%)following treatment with 1μmol·L^-1Guadipyr than by treatment with 10μmol·L^-1 and 100μmol·L^-1.The number of Ca^2+channels activated increased after Guadipyr treatment at a test voltage of -60 mV to+10 mV.The K^+peak current decreased gradually when Guadipyr was added to the external solution;the Ipeak decreased more slowly as the Guadipyr concentration increased from 10μmol·L^-1 to 100μmol·L^-1.Ⅰ-Ⅴcurves declined and the activation of K^+channels was shifted 8 mV towards the depolarizing direction,which was not significant(P>0.05).This shows that K^+channels can be activated at higher potentials by Guadipyr.[Conclusion]Guadipyr can effectively inhibit the Ipeak of the Ca^2+and K^+channels,and shift the activation and inactivation curves of these channels,thereby affecting the gating of Ca^2+channels and K^+channels.Therefore,Ca^2+and K^+ channels in the central neurons of H.armigera are potential Guadipyr target sites.

关 键 词：戊吡虫胍 棉铃虫 电压门控Ca^2+通道 电压门控K^+通道 全细胞膜片钳 

分 类 号：S435.622.3[农业科学—农业昆虫与害虫防治]