碳酸二甲酯三相分配体系萃取分离螺旋藻多糖  

Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of polysaccharides from Spirulina platensis

作　　者：罗光宏[1,2] 雷婕 张喜峰 敬丽娜 王鑫鑫 杨生辉 张青婷[3] LUO Guanghong;LEI Jie;ZHANG Xifeng;JING Lina;WANG Xinxin;YANG Shenghui;ZHANG Qingting(Microalgae Technology Innovation Center of Gansu Province,Hexi University,Zhangye 734000,China;Key Laboratory of Hexi Corridor Resources Utilization of Gansu,Zhangye 734000,China;School of Life Science and Engineering,Hexi University,Zhangye 734000,China)

机构地区：[1]河西学院甘肃省微藻技术创新中心,甘肃张掖734000 [2]甘肃省河西走廊特色资源利用重点实验室,甘肃张掖734000 [3]河西学院生命科学与工程学院,甘肃张掖734000

出　　处：《食品与发酵工业》2020年第17期196-203,共8页Food and Fermentation Industries

基　　金：国家自然科学基金(11665011);甘肃省高校协同创新科技团队支持计划(2017C-17);甘肃省科技计划资助(18JR2JG001);甘肃省重点研发计划项目(18YF1WG087)。

摘　　要：以碳酸二甲酯为有机相,柠檬酸钠为盐相,采用三相分配体系(three-phase partitioning,TPP)对螺旋藻多糖(polysaccharides of Spirulina platensis,PSP)进行萃取分离。结果表明,在碳酸二甲酯与多糖粗提液体积比为0.6∶1.0、柠檬酸钠质量浓度为0.18 g/mL、温度为30℃、pH值为6.0的条件下,制备的螺旋藻多糖(PSP-D)的最大得率为(11.90±0.12)%。PSP-D比叔丁醇和硫酸铵形成TPP制备的多糖(PSP-T)具有更高的多糖含量,PSP-D和PSP-T具有不同的化学组成和分子质量,但其化学结构基本保持不变。PSP-D具有较强的自由基清除能力和总抗氧化能力,因此,碳酸二甲酯作为叔丁醇替代溶剂的TPP体系对天然生物分子的高效分配具有很大的潜力。In this study,a three-phase partitioning(TPP)system with dimethyl carbonate(DMC)as an organic phase and sodium citrate(SC)as salt phase was used for partitioning of polysaccharides from Spirulina platensis(PSP).Results showed that the maximum extraction yield(EY)of PSP-D was(11.90±0.12)%under the modified optimal conditions,DMC to crude extract volume ratio 0.6∶1.0,SC concentration 0.18 g/mL,30℃,and pH 6.0.PSP-D had higher carbohydrate contents compared with the PSP-T,obtained from the TPP system with t-butanol and ammonium sulfate.PSP-D and PSP-T had different chemical compositions and molecular weights;however,their preliminary chemical structures remained unchanged.Moreover,PSP-D exhibited stronger free radical-scavenging capability and total antioxidant capacity than PSP-T.Therefore,the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules.

关 键 词：螺旋藻多糖 三相分配法 碳酸二甲酯 萃取 

分 类 号：TQ281[化学工程—有机化工]