基于叶绿体matK基因序列的两份安康野生桑遗传特征分析  

Genetic character analysis of two wild mulberry varieties in Ankang based on chloroplast matK gene sequence

作　　者：王瑞娴 楚渠[2] 彭云武[2] 孟刚[2] 梁嘉俊 周晓帆 WANG Rui-xian;CHU Qu;PENG Yun-wu;MENG Gang;LIANG Jia-jun;ZHOU Xiao-fan(School of Modern Agriculture and Biotechnology,Ankang University,Ankang 725000,Shaanxi,China;Shaanxi Key Laboratory of Sericulture,Ankang University,Ankang 725000,Shaanxi,China)

机构地区：[1]安康学院现代农业与生物科技学院,陕西安康725000 [2]安康学院陕西省蚕桑重点实验室,陕西安康725000

出　　处：《湖北农业科学》2020年第15期151-154,158,共5页Hubei Agricultural Sciences

基　　金：安康学院校内专项项目(2016AYXNZX019);陕西省教育厅重点实验室科研计划项目(17JS001);安康市科技计划项目(2018AK02-13);大学生创新创业训练计划项目(2017sxjy010)。

摘　　要：对陕西省安康市黄石滩、八仙镇2份野生桑叶绿体基因组进行PCR扩增,获得matK编码基因及其侧翼序列,扩增长度为1715 bp,ORF长度为1518 bp,2份序列仅存在1处单碱基差异。利用matK序列分析7份桑树(Morus alba L.)之间的遗传多样性,结果表明,不同品种桑树matK序列相似度较高,仅存在6个单位点碱基差异,定义3种单倍体,单倍型多样度为0.714,核苷酸多样度为0.00132,平均核苷酸差异为2.000。遗传距离分析表明,八仙镇桑树与印度桑(M.indica)、鲁桑(M.multicaulis)的遗传距离最近,黄石滩桑树与蒙桑(M.mongolica)、华桑(M.cathayana)的遗传距离最近。聚类分析将7份桑树聚为1支,且桑树品种之间的进化支长要明显短于其他科属之间的长度,表明桑树品种间的遗传多样性较低,经历较少进化事件。The matK gene sequences of two wild mulberry that collected form Huangshitan and Baxian town(Ankang city,Shaanxi province)have been obtained by PCR.The length of amplified sequences is 1715 bp while the ORF is 1518 bp.Only one base differ⁃ence when compared with each other.The genetic variations of 7 mulberry have been analyzed with the matK genes.The results showed that 6 variable sites were detected,which defined 3 haplotypes in Morus alba,and the haplotype diversity was 0.714.The nucleotide diversity was 0.00132 and the average nucleotide difference was 2.000 respectively.As for the genetic distances,Baxian was closer to M.indica and M.multicaulis,while Huangshitan was closer to M.mongolica and M.cathayana.Cluster analysis demonstrated that the seven mulberries were grouped together,and the branch was shorter than other species,implied lower genetic diversity and fewer evo⁃lutionary events proceeded.

关 键 词：桑树(Morus alba L.) 进化分析 遗传多样性 叶绿体 MATK 

分 类 号：S888.2[农业科学—特种经济动物饲养]