玉米矮杆突变体A5的表型鉴定及转录组分析  

Phenotypic Characterization and Transcriptome Analysis of Maize Dwarf Mutant A5

作　　者：杨睿 张正 杨丽莉 张彦琴 董春林 常建忠 YANG Rui;ZHANG Zheng;YANG Lili;ZHANG Yanqin;DONG Chunlin;CHANG Jianzhong(Shanti Academy of Organic Dryland Agriculture,National Local Joint Engineering Laboratory of Water saving Techniques for Dry Farming in the Eastern Loess Plateau,Organic Dry Farming of Shanxi Province Key Laboratory,Shanxi Agricultural University.Taiyuan 030031,China)

机构地区：[1]山西农业大学山西有机旱作农业研究院,黄土高原东部旱作节水技术国家地方联合工程实验室,有机旱作山西省重点实验室,山西太原030031

出　　处：《山西大学学报（自然科学版）》2020年第3期597-603,共7页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省重点研发计划项目(201903D221078);山西省农业科学院农业科技创新研究项目(YCX2020YQ54)。

摘　　要：玉米矮化植株株型紧凑、抗倒伏、耐密植,能够提高光能利用率,从而提高产量。文章对源自PH6WC的玉米矮杆突变体A5进行了表型鉴定、遗传分析和转录组分析。结果表明突变体A5株高较野生型显著降低37.99%,其矮化表型由节间长度缩短所致;遗传分析表明A5矮化表型受一对隐性单基因控制,外源GA3不能彻底恢复其矮杆表型;转录组分析共鉴定出2 040个差异表达基因,包含上调表达基因708个和下调表达基因1 332个,其中有赤霉素合成相关基因d1(Zm00001d039634)和ga2ox(Zm00001d037565)及细胞分裂相关基因tan1(Zm00001d038060)等。The maize dwarf plant,with compact plant type,great lodging and density resistance,could greatly improve utilization rate of solar energy and increase the production.We performed phenotypic identification,genetic and transcriptome analysis on EMS-induced dwarf mutant A5 derived from PH6 WC.The results showed that the plant-height of A5 was 37.99%shorter than PH6 WC and the dwarf phenotype attributed to the shortened internodes length.The genetic analysis indicated that the mutant phenotype of A5 was controlled by a single recessive gene and exogenous GA3 cannot completely recover its dwarf phenotype.The transcriptome analysis identified 2040 differentially expressed genes in mutant A5,including 708 up-regulated genes and 1332 down-regulated genes.Among them,some of the gibberellins synthesis-related and cell division-related genes were observed,such as d1(Zm00001 d039634),ga2ox(Zm00001 d037565),tan1(Zm00001 d038060)and etc.

关 键 词：玉米 矮杆突变体 表型鉴定 赤霉素 转录组分析 

分 类 号：S513.035.3[农业科学—作物学]