亚临床甲状腺功能减退合并妊娠期糖尿病孕妇血清核因子κB及白细胞介素水平的变化  

Changes of serum nuclear factor κB and interleukins in pregnant women with subclinical hypothyroid complicated with gestational diabetes mellitus

作　　者：郑智君[1] 乔国昱 侯静 温芳 肖凤艳[3] 郑建霞[3] Zheng Zhijun;Qiao Guoyu;Hou Jing;Wen Fang;Xiao Fengyan;Zheng Jianxia(Department of Clinical Laboratory,The Second Hospital of Tangshan,Hebei Province,Tangshan 063000,China;Department of Clinical Laboratory,Tangshan Union Medical College Hospital,Hebei Province,Tangshan 063000,China;Department of Clinical Laboratory,Tangshan Female and Child Health Hospital,Hebei Province,Tangshan 063000,China)

机构地区：[1]河北省唐山市第二医院检验科,063000 [2]河北省唐山市协和医院检验科,063000 [3]河北省唐山市妇幼保健院检验科,063000

出　　处：《中国综合临床》2020年第5期389-393,共5页Clinical Medicine of China

基　　金：河北省医学科学研究重点课题计划项目(20171311)。

摘　　要：目的探讨亚临床甲状腺功能减退合并妊娠期糖尿病孕妇血清核因子κB、白细胞介素(interleukin,IL)4、IL-10、IL-12、干扰素γ表达的临床意义。方法采用回顾性分析方法,选取2017年1月至2018年10月在唐山市妇幼保健院定期产检的亚临床甲状腺功能减退合并妊娠期糖尿病孕妇30例为A组,另选同期单纯亚临床甲状腺功能减退孕妇33例为B组,单纯妊娠期糖尿病孕妇35例为C组,健康体检孕妇40名为对照组。所有研究对象均采用酶联免疫法检测血清核因子κB、IL-4、IL-10、IL-12和干扰素γ,并对结果进行分析比较。结果A、B、C组及对照组血清核因子κB分别为(15.91±5.68)、(13.22±5.23)、(12.97±5.11)、(9.74±3.85)μg/L,血清IL-12分别为(28.91±6.84)、(21.64±5.72)、(22.23±5.91)、(13.68±3.76)ng/L、血清干扰素γ分别为(23.74±5.55)、(18.26±4.63)、(17.85±4.31)、(12.69±3.85)ng/L,3项指标组间比较差异均有统计学意义(F值分别为5.118、6.821、7.133,P均<0.05),且A组均高于B、C组及对照组,差异均有统计学意义(P均<0.05);B、C组也高于对照组,差异均有统计学意义(P均<0.05);A、B、C组及对照组血清IL-4分别为(8.91±3.99)、(10.84±4.47)、(11.27±4.62)、(13.68±5.46)ng/L,血清IL-10分别为(10.91±3.86)、(13.05±4.58)、(12.83±4.69)、(15.82±5.33)ng/L,血清IL-4、IL-10组间比较差异均有统计学意义(F值分别5.075、5.616,P均<0.05),且A组血清IL-4、IL-10低于B、C组及对照组,B组和C组血清IL-4、IL-10低于对照组,差异均有统计学意义(P均<0.05)。结论核因子κB信号通路活化及相关的细胞因子可能是促进亚临床甲状腺功能减退合并妊娠期糖尿病发生的影响因素。Objective To explore the clinical significance of serum nuclear factorκB,interleukin(IL)-4,IL-10,IL-12,interferon(IFN)-γexpression in subclinical hypothyroidism with gestational diabetes mellitus.Methods Thirty pregnant women with subclinical hypothyroidism combined with gestational diabetes mellitus in Tangshan Maternal and Child Health Hospital from January 2017 to October 2018 were retrospectively analyzed as group A.Thirty three pregnant women with subclinical hypothyroidism were selected as group B,35 pregnant women with gestational diabetes mellitus as group C and 40 healthy pregnant women as control group.ELISA was used to detect NF-κB,IL-4,IL-10,IL-12 and IFN-γ,and the results were analyzed and compared.Results The serum levels of NF-κB were(15.91±5.68),(13.22±5.23),(12.97±5.11),(9.74±3.85)μg/L,IL-12 were(28.91±6.84),(21.64±5.72),(22.23±5.91),(13.68±3.76)ng/L,and serum IFN-γlevels were(23.74±5.55),(18.26±4.63),(17.85±4.31),(12.69±3.85)ng/L in A,B,C and the control group respectively.There were statistically significant differences in the three indicators between groups(F=5.118,6.821,7.133,all P<0.05),and group A was higher than group B,C and control,the differences were statistically significant(all P<0.05);the levels of serum IL-4 in group A,B,C and control group were(8.91±3.99),(10.84±4.47),(11.27±4.62),(13.68±5.46)ng/L,respectively.The levels of serum IL-10 were(10.91±3.86),(13.05±4.58),(12.83±4.69),(15.82±5.33)ng/L,respectively.The differences of serum IL-4 and IL-10 between groups were statistically significant(F=5.075,5.616,all P<0.05),and serum IL-4 and IL-10 in group A were lower than those in group B,group C and control group.The levels of serum IL-4 and IL-10 in group B and C were lower than those in control group(all P<0.05).Conclusion The activation of NF-kB signaling pathway and its related cytokines may be the influencing factors for the development of subclinical hypothyroidism with gestational diabetes mellitus.

关 键 词：亚临床甲状腺功能减退 糖尿病 妊娠期 核因子ΚB 白细胞介素4 白细胞介素10 白细胞介素12 干扰素Γ 

分 类 号：R714.256[医药卫生—妇产科学]