桔小实蝇flightin基因RNAi载体构建  

Construction of Interference Vector of Bactrocera dorsalis(Hendel)flightin Gene

作　　者：袁瑞玲 郑传伟 冯丹 王艺璇[1] 杜春花 陈鹏 YUAN Rui-ling;ZHENG Chuan-wei;FENG Dan;WANG Yi-xuan;DU Chun-hua;CHEN Peng(Yunnan Academy of Forestry and Grassland,Kunming Yunnan 650201,P.R.China;Xingyi City Forestry Bureau,Xingyi Guizhou 562400,P.R.China;Key Laboratory of Forest Plant Cultivation&Utilization,Kunming Yunnan 650201,P.R.China)

机构地区：[1]云南省林业和草原科学院,云南昆明650201 [2]贵州省兴义市林业局,贵州兴义562400 [3]云南省森林植物培育与开发利用重点实验室,云南昆明650201

出　　处：《西部林业科学》2020年第4期171-175,共5页Journal of West China Forestry Science

基　　金：国家自然科学基金项目(31660208)。

摘　　要：以云南省林业和草原科学院森保所室内饲养的桔小实蝇为材料,采用RT-PCR技术,克隆桔小实蝇(Bactrocera dorsalis Hendel.)flightin的基因片段,并以此为靶标,设计有效的干扰片段,以线虫RNAi干扰载体L4440为载体,构建桔小实蝇flightin基因RNAi载体。通过PCR、酶切检测及测序,证明表达载体构建正确。本研究为通过RNAi研究桔小实蝇flightin的基因功能奠定了基础。The sequence of flightin in Bactrocera dorsalis(Hendel)was cloned in this study by RT-PCR.This flightin sequence was chosen as the target gene.An interference fragment was designed and inserted into the expression vector L4440.Restriction enzyme analysis and DNA sequencing showed that all the recombinant plasmids were according with our design.Our study laid a foundation for the study of the function of flightin gene in B.dorsalis by RNAi.

关 键 词：桔小实蝇 flightin基因 L4440载体 RNA干扰 

分 类 号：Q966[生物学—昆虫学]