管碟法测定抗生素效价影响因素与控制方法的研究进展  

Research progress of the influencing factors and control methods for the determination of antibiotic potency by the cylinder-plate method

作　　者：任仲丽 张秀花 王静 李秀菊 刘博 REN Zhong-li;ZHANG Xiu-hua;WANG Jing;LI Xiu-ju;LIU Bo(Institute of Biological Detection,Food and Drug Inspection and Testing Institute of Heze City,Shandong Province,Heze274000,China)

机构地区：[1]菏泽市食品药品检验检测研究院生物检测研究所,山东菏泽274000

出　　处：《中国当代医药》2020年第25期27-29,33,共4页China Modern Medicine

摘　　要：管碟法是目前国内外常用的抗生素微生物检定法,方法经典成熟,灵敏度高,能直观地反映抗生素药品的抗菌活性。本文通过查阅资料及实际操作经验总结,对管碟法测定抗生素效价的影响因素进行分析、归纳,并提出合理有效的解决方案,旨在减少外界与人为因素引起的误差,使测定结果与真实值更为接近,使试验的准确性得到有效提高。The cylinder-plate method is a commonly used method for microbiological assay of antibiotics at home and abroad.The method is classical and mature,with high sensitivity,and can directly reflect the antibacterial activity of antibiotics.This paper analyzes and summarizes the factors influencing on the assay of antibiotic microbiological by cylinder-plate method through data review and practical experience summary,and puts forward reasonable and effective solutions to reduce the errors caused by external and human factors,so that the measurement results are closer to the real value and the accuracy of the test is effectively improved.

关 键 词：管碟法 抗生素效价 影响因素 准确性 

分 类 号：R927.1[医药卫生—药学]